Anti-IKKEpsilon Antibody (CAB14093)
- SKU:
- CAB14093
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| Antibody Name: | IKK? Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB14093 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 495-716 of human IKK? (NP_054721.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | K-562, 293T, SW480, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 495-716 of human IKK? (NP_054721.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LRSR LRTL AEVL SRCS QNIT ETQE SLSS LNRE LVKS RDQV HEDR SIQQ IQCC LDKM NFIY KQFK KSRM RPGL GYNE EQIH KLDK VNFS HLAK RLLQ VFQE ECVQ KYQA SLVT HGKR MRVV HETR NHLR LVGC SVAA CNTE AQGV QESL SKLL EELS HQLL QDRA KGAQ ASPP PIAP YPSP TRKD LLLH MQEL CEGM KLLA SDLL DNNR IIER LNRV PAPP DV |
| Gene ID: | 9641 |
| Uniprot: | Q14164 |
| Cellular Location: | Cytoplasm, Nucleus, PML body |
| Calculated MW: | 70kDa/80kDa |
| Observed MW: | 80kDa |
| Synonyms: | IKBKE, IKK-E, IKK-i, IKKE, IKKI |
| Background: |
| UniProt Protein Function: | IKKE: a oncogenic kinase of the IKK family. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Plays a pivotal role in coordinating the activation of IRF3 and NF-kappaB in the innate immune response. Its redistribution to PML nuclear bodies upon DNA damage is TOPORS-dependent. Is a breast cancer oncogene that exerts its oncogenic properties through cRel. |
| UniProt Protein Details: | Protein type:Inhibitor; Kinase, protein; EC 2.7.11.10; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Other group; IKK family Chromosomal Location of Human Ortholog: 1q32.1 Cellular Component: nucleoplasm; PML body; cytoplasm; mitochondrial membrane; endosome membrane; nucleus; cytosol Molecular Function:NF-kappaB-inducing kinase activity; protein binding; IkappaB kinase activity; ATP binding Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; DNA damage response, signal transduction resulting in induction of apoptosis; MyD88-independent toll-like receptor signaling pathway; toll-like receptor signaling pathway; innate immune response; immune response; toll-like receptor 3 signaling pathway; I-kappaB phosphorylation; negative regulation of interferon type I production; toll-like receptor 4 signaling pathway; protein amino acid phosphorylation |
| NCBI Summary: | IKBKE is a noncanonical I-kappa-B (see MIM 164008) kinase (IKK) that is essential for regulating antiviral signaling pathways. IKBKE has also been identified as a breast cancer (MIM 114480) oncogene and is amplified and overexpressed in over 30% of breast carcinomas and breast cancer cell lines (Hutti et al., 2009 [PubMed 19481526]).[supplied by OMIM, Oct 2009] |
| UniProt Code: | Q14164 |
| NCBI GenInfo Identifier: | 14548079 |
| NCBI Gene ID: | 9641 |
| NCBI Accession: | Q14164.1 |
| UniProt Secondary Accession: | Q14164,Q3B754, Q3KR43, Q5JTS6, D3DT78, |
| UniProt Related Accession: | Q14164 |
| Molecular Weight: | 716 |
| NCBI Full Name: | Inhibitor of nuclear factor kappa-B kinase subunit epsilon |
| NCBI Synonym Full Names: | inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon |
| NCBI Official Symbol: | IKBKE |
| NCBI Official Synonym Symbols: | IKKE; IKKI; IKK-E; IKK-i |
| NCBI Protein Information: | inhibitor of nuclear factor kappa-B kinase subunit epsilon; IKK-epsilon; I-kappa-B kinase epsilon; inducible IkappaB kinase; IKK-related kinase epsilon; inducible I kappa-B kinase |
| UniProt Protein Name: | Inhibitor of nuclear factor kappa-B kinase subunit epsilon |
| UniProt Synonym Protein Names: | Inducible I kappa-B kinase; IKK-i |
| UniProt Gene Name: | IKBKE |
| UniProt Entry Name: | IKKE_HUMAN |
 | Western blot analysis of extracts of various cell lines, using IKKe antibody (CAB14093) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded rat spleen using IKKe antibody (CAB14093) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using IKKe antibody (CAB14093) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of C6 cells using IKKe antibody (CAB14093) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of HeLa cells using IKKe antibody (CAB14093) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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