Anti-IGFBP5 Antibody (CAB12451)
- SKU:
- CAB12451
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-IGFBP5 Antibody |
| Antibody SKU: | CAB12451 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 68-167 of human IGFBP5 (NP_000590.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, BT-474, Mouse kidney, Mouse uterus, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 68-167 of human IGFBP5 (NP_000590.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | GVYT ERCA QGLR CLPR QDEE KPLH ALLH GRGV CLNE KSYR EQVK IERD SREH EEPT TSEM AEET YSPK IFRP KHTR ISEL KAEA VKKD RRKK LTQS KFVG |
| Gene ID: | 3488 |
| Uniprot: | P24593 |
| Cellular Location: | Secreted |
| Calculated MW: | 30kDa |
| Observed MW: | 30kDa |
| Synonyms: | IGFBP5, IBP5 |
| Background: |
| UniProt Protein Function: | IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. |
| UniProt Code: | P24593 |
| NCBI GenInfo Identifier: | 124069 |
| NCBI Gene ID: | 3488 |
| NCBI Accession: | P24593.1 |
| UniProt Secondary Accession: | P24593,Q5U0A3, |
| UniProt Related Accession: | P24593 |
| Molecular Weight: | |
| NCBI Full Name: | Insulin-like growth factor-binding protein 5 |
| NCBI Synonym Full Names: | insulin like growth factor binding protein 5 |
| NCBI Official Symbol: | IGFBP5 |
| NCBI Official Synonym Symbols: | IBP5 |
| NCBI Protein Information: | insulin-like growth factor-binding protein 5 |
| UniProt Protein Name: | Insulin-like growth factor-binding protein 5 |
| Protein Family: | Insulin-like growth factor-binding protein |
| UniProt Gene Name: | IGFBP5 |
 | Western blot analysis of extracts of various cell lines, using IGFBP5 antibody (CAB12451) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded mouse brain using IGFBP5 antibody (CAB12451) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat brain using IGFBP5 antibody (CAB12451) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using IGFBP5 antibody (CAB12451) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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