Anti-IDH2 Antibody (CAB7190)
- SKU:
- CAB7190
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-IDH2 Antibody |
| Antibody SKU: | CAB7190 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF IP ChIP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 193-452 of human IDH2 (NP_002159.2). |
| Application: | WB IHC IF IP ChIP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:20 - 1:50 IP 1:50 - 1:200 ChIP 1:20 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HL-60, MCF-7, Jurkat, HepG2, U-87MG, Mouse kidney, Mouse heart, Rat lung, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 193-452 of human IDH2 (NP_002159.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | KMVF TPKD GSGV KEWE VYNF PAGG VGMG MYNT DESI SGFA HSCF QYAI QKKW PLYM STKN TILK AYDG RFKD IFQE IFDK HYKT DFDK NKIW YEHR LIDD MVAQ VLKS SGGF VWAC KNYD GDVQ SDIL AQGF GSLG LMTS VLVC PDGK TIEA EAAH GTVT RHYR EHQK GRPT STNP IASI FAWT RGLE HRGK LDGN QDLI RFAQ MLEK VCVE TVES GAMT KDLA GCIH GLSN VKLN EHFL NTTD FLDT IKSN LDRA LGRQ |
| Gene ID: | 3418 |
| Uniprot: | P48735 |
| Cellular Location: | Mitochondrion |
| Calculated MW: | 45kDa/50kDa |
| Observed MW: | 51kDa |
| Synonyms: | IDH2, D2HGA2, ICD-M, IDH, IDHM, IDP, IDPM, mNADP-IDH |
| Background: | Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | IDH2: a mitochondrial oxidoreductase that catalyzes the third step of the citric acid cycle: the oxidative decarboxylation of isocitrate, consuming NADP(+), and producing alpha-ketoglutarate (alpha-KG) and CO2. Can also use oxalosuccinate as a substrate. Alpha-KG is an activator the dioxygenases that hydroxylate the transcription factor HIF and lead to its degradation by VHL. Since HIF turns on oncogenic pathways, IDH2 has apparent tumor suppressor activity. Homo-dimerization is required for activity. Each subunit binds 1 magnesium or manganese ion. May tightly associate or interact with the pyruvate dehydrogenase complex. A somatic mutation resulting in the R172K conversion results in a neomorphic enzyme activity that converts isocitrate to R(-)-2-hydroxyglutarate (2HG) occurs in a significant percentage of patients with cytogenetically normal acute myeloid leukemia (AML). Elevated levels of 2HG are also correlated with an elevated risk of malignant brain tumors. |
| UniProt Protein Details: | Protein type:Mitochondrial; Carbohydrate Metabolism - citrate (TCA) cycle; EC 1.1.1.42; Other Amino Acids Metabolism - glutathione; Oxidoreductase Chromosomal Location of Human Ortholog: 15q26.1 Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane Molecular Function:magnesium ion binding; isocitrate dehydrogenase (NADP+) activity; NAD binding Biological Process: glyoxylate cycle; isocitrate metabolic process; cellular metabolic process; tricarboxylic acid cycle; carbohydrate metabolic process; 2-oxoglutarate metabolic process Disease: D-2-hydroxyglutaric Aciduria 2 |
| NCBI Summary: | Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014] |
| UniProt Code: | P48735 |
| NCBI GenInfo Identifier: | 20141568 |
| NCBI Gene ID: | 3418 |
| NCBI Accession: | P48735.2 |
| UniProt Secondary Accession: | P48735,Q96GT3, B2R6L6, B4DFL2, |
| UniProt Related Accession: | P48735 |
| Molecular Weight: | 452 |
| NCBI Full Name: | Isocitrate dehydrogenase |
| NCBI Synonym Full Names: | isocitrate dehydrogenase 2 (NADP+), mitochondrial |
| NCBI Official Symbol: | IDH2 |
| NCBI Official Synonym Symbols: | IDH; IDP; IDHM; IDPM; ICD-M; D2HGA2; mNADP-IDH |
| NCBI Protein Information: | isocitrate dehydrogenase [NADP], mitochondrial; isocitrate dehydrogenase [NADP], mitochondrial; NADP(+)-specific ICDH; oxalosuccinate decarboxylase |
| UniProt Protein Name: | Isocitrate dehydrogenase [NADP], mitochondrial |
| UniProt Synonym Protein Names: | ICD-M; IDP; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase |
| Protein Family: | Isocitrate dehydrogenase |
| UniProt Gene Name: | IDH2 |
| UniProt Entry Name: | IDHP_HUMAN |
 | Western blot analysis of extracts of various cell lines, using IDH2 antibody (CAB7190) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human colon carcinoma using IDH2 antibody (CAB7190) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of C6 cells using IDH2 antibody (CAB7190) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using IDH2 antibody (CAB7190) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 200ug extracts of MCF7 cells using 1ug IDH2 antibody (CAB7190). Western blot was performed from the immunoprecipitate using IDH2 antibody (CAB7190) at a dilition of 1:1000. |
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