Anti-ICT1 Antibody (CAB11590)
- SKU:
- CAB11590
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-ICT1 Antibody |
| Antibody SKU: | CAB11590 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-206 of human ICT1 (NP_001536.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Raji, HT-29, 293T, B cells, SKOV3, Mouse lung, Mouse kidney, Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-206 of human ICT1 (NP_001536.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LHKQ KDGT EFKS IYSL DKLY PESQ GSDT AWRV PNGA KQAD SDIP LDRL TISY CRSS GPGG QNVN KVNS KAEV RFHL ATAE WIAE PVRQ KIAI THKN KINR LGEL ILTS ESSR YQFR NLAD CLQK IRDM ITEA SQTP KEPT KEDV KLHR IRIE NMNR ERLR QKRI HSAV KTSR RVDM D |
| Gene ID: | 3396 |
| Uniprot: | Q14197 |
| Cellular Location: | Mitochondrion |
| Calculated MW: | 23kDa |
| Observed MW: | 24kDa |
| Synonyms: | MRPL58, DS-1, DS1, ICT1, MRP-L58 |
| Background: | The protein encoded by this gene is a peptidyl-tRNA hydrolase and a vital component of the large mitochondrial ribosome. The encoded protein serves as a ribosome release factor for this ribosome, which translates mitochondrial genes. This protein may be responsible for degrading prematurely-terminated polypeptides and for reusing stalled ribosomes. Two transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | ICT1: Essential peptidyl-tRNA hydrolase component of the mitochondrial large ribosomal subunit. Acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion, possibly in case of abortive elongation. May be involved in the hydrolysis of peptidyl-tRNAs that have been prematurely terminated and thus in the recycling of stalled mitochondrial ribosomes. Belongs to the prokaryotic/mitochondrial release factor family. ICT1 subfamily. |
| UniProt Protein Details: | Protein type:EC 3.1.1.29; Mitochondrial Chromosomal Location of Human Ortholog: 17q25.1 Cellular Component: mitochondrial inner membrane; mitochondrial large ribosomal subunit; mitochondrial matrix; mitochondrion Molecular Function:aminoacyl-tRNA hydrolase activity; translation release factor activity, codon nonspecific |
| NCBI Summary: | The protein encoded by this gene is a peptidyl-tRNA hydrolase and a vital component of the large mitochondrial ribosome. The encoded protein serves as a ribosome release factor for this ribosome, which translates mitochondrial genes. This protein may be responsible for degrading prematurely-terminated polypeptides and for reusing stalled ribosomes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2014] |
| UniProt Code: | Q14197 |
| NCBI GenInfo Identifier: | 6015033 |
| NCBI Gene ID: | 3396 |
| NCBI Accession: | Q14197.1 |
| UniProt Secondary Accession: | Q14197,Q53HM7, Q53Y11, B2RAD1, |
| UniProt Related Accession: | Q14197 |
| Molecular Weight: | 24kDa |
| NCBI Full Name: | Peptidyl-tRNA hydrolase ICT1, mitochondrial |
| NCBI Synonym Full Names: | mitochondrial ribosomal protein L58 |
| NCBI Official Symbol: | MRPL58 |
| NCBI Official Synonym Symbols: | DS1; DS-1; ICT1; MRP-L58 |
| NCBI Protein Information: | peptidyl-tRNA hydrolase ICT1, mitochondrial |
| UniProt Protein Name: | Peptidyl-tRNA hydrolase ICT1, mitochondrial |
| UniProt Synonym Protein Names: | 39S ribosomal protein L58, mitochondrial |
| Protein Family: | 1-acylglycerol-3-phosphate O-acyltransferase |
| UniProt Gene Name: | MRPL58 |
 | Western blot analysis of extracts of various cell lines, using ICT1 antibody (CAB11590) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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