Anti-ICMT Antibody (CAB10293)
- SKU:
- CAB10293
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-ICMT Antibody |
| Antibody SKU: | CAB10293 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 175-284 of human ICMT (NP_036537.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, K-562, HeLa, THP-1, Mouse testis, Mouse lung, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 175-284 of human ICMT (NP_036537.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | KAAM FTAG SNFN HVVQ NEKS DTHT LVTS GVYA WFRH PSYV GWFY WSIG TQVM LCNP ICGV SYAL TVWR FFRD RTEE EEIS LIHF FGEE YLEY KKRV PTGL PFIK GVKV DL |
| Gene ID: | 23463 |
| Uniprot: | O60725 |
| Cellular Location: | Endoplasmic reticulum membrane, Multi-pass membrane protein |
| Calculated MW: | 31kDa |
| Observed MW: | 32kDa |
| Synonyms: | ICMT, HSTE14, MST098, MSTP098, PCCMT, PCMT, PPMT |
| Background: | This gene encodes the third of three enzymes that posttranslationally modify isoprenylated C-terminal cysteine residues in certain proteins and target those proteins to the cell membrane. This enzyme localizes to the endoplasmic reticulum. Alternative splicing may result in other transcript variants, but the biological validity of those transcripts has not been determined. |
| UniProt Protein Function: | ICMT: Catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues. Belongs to the isoprenylcysteine O-methyltransferase family. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Methyltransferase; EC 2.1.1.100; Transcription, coactivator/corepressor; Endoplasmic reticulum; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 1p36.21 Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; integral to membrane; membrane Molecular Function:C-terminal protein carboxyl methyltransferase activity; protein-S-isoprenylcysteine O-methyltransferase activity Biological Process: C-terminal protein amino acid methylation; post-translational protein modification; protein modification process; protein targeting to membrane; S-adenosylhomocysteine metabolic process; S-adenosylmethioninamine metabolic process |
| NCBI Summary: | This gene encodes the third of three enzymes that posttranslationally modify isoprenylated C-terminal cysteine residues in certain proteins and target those proteins to the cell membrane. This enzyme localizes to the endoplasmic reticulum. Alternative splicing may result in other transcript variants, but the biological validity of those transcripts has not been determined. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O60725 |
| NCBI GenInfo Identifier: | 14548077 |
| NCBI Gene ID: | 23463 |
| NCBI Accession: | O60725.1 |
| UniProt Secondary Accession: | O60725,Q6FHT0, |
| UniProt Related Accession: | O60725 |
| Molecular Weight: | 31,938 Da |
| NCBI Full Name: | Protein-S-isoprenylcysteine O-methyltransferase |
| NCBI Synonym Full Names: | isoprenylcysteine carboxyl methyltransferase |
| NCBI Official Symbol: | ICMT |
| NCBI Official Synonym Symbols: | PCMT; PPMT; PCCMT; HSTE14; MST098; MSTP098 |
| NCBI Protein Information: | protein-S-isoprenylcysteine O-methyltransferase |
| UniProt Protein Name: | Protein-S-isoprenylcysteine O-methyltransferase |
| UniProt Synonym Protein Names: | Isoprenylcysteine carboxylmethyltransferase; Prenylated protein carboxyl methyltransferase; PPMT; Prenylcysteine carboxyl methyltransferase; pcCMT |
| Protein Family: | CMT1A duplicated region transcript 1 protein |
| UniProt Gene Name: | ICMT |
| UniProt Entry Name: | ICMT_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ICMT antibody (CAB10293) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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