Anti-IARS Antibody (CAB10190)
- SKU:
- CAB10190
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-IARS Antibody |
| Antibody SKU: | CAB10190 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1158-1262 of human IARS (NP_002152.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, HepG2, HT-29, HL-60, Jurkat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1158-1262 of human IARS (NP_002152.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SAPS LINS SSTL LCQY INLQ LLNA KPQE CLMG TVGT LLLE NPLG QNGL THQG LLYE AAKV FGLR SRKL KLFL NETQ TQEI TEDI PVKT LNMK TVYV SVLP TTAD F |
| Gene ID: | 3376 |
| Uniprot: | P41252 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 144kDa |
| Observed MW: | 144kDa |
| Synonyms: | IARS, GRIDHH, IARS1, ILERS, ILRS, IRS, PRO0785 |
| Background: | Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Isoleucine-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family and has been identified as a target of autoantibodies in the autoimmune disease polymyositis/dermatomyositis. Alternatively spliced transcript variants have been found. |
| UniProt Protein Function: | IARS: Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Isoleucine-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family and has been identified as a target of autoantibodies in the autoimmune disease polymyositis/dermatomyositis. Two alternatively spliced variants have been isolated that represent alternate 5' UTRs. [provided by RefSeq, Jul 2008] |
| UniProt Protein Details: | Protein type:Amino Acid Metabolism - valine, leucine and isoleucine biosynthesis; Aminoacyl-tRNA synthetase; EC 6.1.1.5; Ligase; Translation; Translation regulation Chromosomal Location of Human Ortholog: 9q22.31 Cellular Component: cytoplasm; cytosol; membrane Molecular Function:GTPase binding; isoleucine-tRNA ligase activity; protein binding Biological Process: isoleucyl-tRNA aminoacylation; osteoblast differentiation; tRNA aminoacylation for protein translation Disease: Growth Retardation, Intellectual Developmental Disorder, Hypotonia, And Hepatopathy |
| NCBI Summary: | Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Isoleucine-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family and has been identified as a target of autoantibodies in the autoimmune disease polymyositis/dermatomyositis. Alternatively spliced transcript variants have been found. [provided by RefSeq, Nov 2012] |
| UniProt Code: | P41252 |
| NCBI GenInfo Identifier: | 239938717 |
| NCBI Gene ID: | 3376 |
| NCBI Accession: | P41252.2 |
| UniProt Secondary Accession: | P41252,Q5TCD0, Q7Z3T4, Q9H588, A8KAE9, |
| UniProt Related Accession: | P41252 |
| Molecular Weight: | 144kDa |
| NCBI Full Name: | Isoleucine--tRNA ligase, cytoplasmic |
| NCBI Synonym Full Names: | isoleucyl-tRNA synthetase |
| NCBI Official Symbol: | IARS |
| NCBI Official Synonym Symbols: | IRS; ILRS; IARS1; ILERS; GRIDHH; PRO0785 |
| NCBI Protein Information: | isoleucine--tRNA ligase, cytoplasmic |
| UniProt Protein Name: | Isoleucine--tRNA ligase, cytoplasmic |
| UniProt Synonym Protein Names: | Isoleucyl-tRNA synthetase; IRS; IleRS |
| UniProt Gene Name: | IARS |
 | Western blot analysis of extracts of various cell lines, using IARS antibody (CAB10190) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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