Anti-Human/Mouse Integrin β7 In Vivo Antibody - Low Endotoxin

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SKU:
IVMB0087
Product Type:
In Vivo Monoclonal Antibody
Clone:
FIB504
Protein:
Integrin Beta 7
Isotype:
Rat IgG2a kappa
Reactivity:
Human
Mouse
Synonyms:
beta-7 Integrin
integrin beta-p
ITGB7
Research Area:
Adhesion & Migration
Endotoxin Level:
Low Endotoxin
Host Species:
Rat
Applications:
Blocking
CyTOF
FC
IP
Frequently bought together:

Description

system_update_altTechnical Datasheet
Product Name:Anti-Human/Mouse Integrin β7 In Vivo Antibody - Low Endotoxin
Product Code:IVMB0087
Size:1mg, 5mg, 25mg, 50mg, 100mg
Clone:FIB504
Protein:Integrin Beta 7
Product Type:Monoclonal Antibody
Synonyms:beta-7 Integrin, integrin beta-p, ITGB7
Isotype:Rat IgG2a κ
Reactivity:Human/Mouse
Immunogen:TK1 Cells
Applications:B, CyTOF®, FC, IP
Formulation: This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:< 1.0 EU/mg as determined by the LAL method
Purity: ≥95% monomer by analytical SEC
>95% by SDS Page
Preparation: Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling: Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Applications:B, CyTOF®, FC, IP
Recommended Usage: FC
The suggested concentration of use for clone FIB504 antibody for staining cells in flow cytometry is ≤0.5 µg per 106 in a volume of 100 µl or 100 µl of whole blood. Each lot of this antibody is quality control tested.
Reactivity:Human/Mouse
Host Species:Rat
Specificity:Clone FIB504 recognizes an epitope on human/mouse integrin beta-7.
Antigen Distribution: Integrin α4beta-7 is expressed on subsets of peripheral lymphocytes, thymocytes, and bone marrow progenitors. The αEbeta-7 integrin is expressed on mucosal T cells, including intraepithelial T lymphocytes (IELs) and lamina propria T cells, subsets of dendritic cells, and regulatory T cells (Tregs)8.
Immunogen:TK1 Cells
Concentration:≥ 5.0 mg/ml
Endotoxin Level:< 1.0 EU/mg as determined by the LAL method
Purity: ≥95% monomer by analytical SEC
>95% by SDS Page
Formulation: This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation: Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling: Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Integrin β7 antibody, clone FIB504, recognizes integrin β7, a 130 kDa membrane glycoprotein of the Ig superfamily. Integrin β7 forms heterodimers with both the α4 (CD49d) and aE (CD103) integrins. α4β7 integrin is expressed on subsets of peripheral lymphocytes, thymocytes, and bone marrow progenitors1,2. It mediates adhesion to mucosal endothelial cells, promoting leukocyte transendothelial migration through interactions with mucosal addressin cell adhesion molecule-1 (MAdCAM-1)3,4. The αEβ7 integrin is expressed on mucosal T cells, including intraepithelial T lymphocytes (IELs) and lamina propria T cells5,6, subsets of dendritic cells7, and regulatory T cells (Tregs)8. It facilitates retention in the gut epithelial layer via interactions with E-cadherin9. Anti-β7 antibodies block both the homing to and retention in the gut of pathogenic T cells10 and are currently under evaluation in a phase 3 clinical trial to treat inflammatory bowel disease11.

Technical Datasheet:View
Protein:Integrin Beta 7
Function:Lymphocyte adhesion, hematopoietic progenitor cells migration
Ligand/Receptor: CD49d/beta-7: VCAM-1 (CD106), MAdCAM-1 and fibronectin; CD103/beta-7: E-cadherin
Research Area:Cell Biology, Immunology, Neuroscience
Product Thumbnail
Rat IgG2a Isotype Control
Clone 1-1
Isotype Rat IgG2a
Endotoxin Level Low Endotoxin
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