Anti-Human HLA-DP (MHC Class II) Monomorphic In Vivo Antibody - Low Endotoxin
- SKU:
- IVMB0131
- Product Type:
- In Vivo Monoclonal Antibody
- Clone:
- B7/21
- Protein:
- HLA-DP Monomorphic
- Isotype:
- Mouse IgG3
- Reactivity:
- Human
- Synonyms:
- HLA-DP Monomorphic
- HLADP
- HLA-DP alpha beta
- DPA and DPB
- DPalpha and DPbeta
- Dpalpha beta
- Endotoxin Level:
- Low Endotoxin
- Host Species:
- Mouse
- Applications:
- FC
- ICC
Description
| Product Name: | Anti-Human HLA-DP (MHC Class II) Monomorphic In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0131 |
| Size: | 1mg, 5mg, 25mg, 50mg |
| Clone: | B7/21 |
| Protein: | HLA-DP Monomorphic |
| Product Type: | Monoclonal Antibody |
| Synonyms: | HLA-DP Monomorphic, HLADP, HLA-DPαβ, DPA and DPB, DPα and DPβ, DPαβ |
| Isotype: | Mouse IgG3 |
| Reactivity: | Human |
| Immunogen: | Unknown |
| Applications: | FC, ICC |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. Note: B7/21 is an antibody prone to precipitation at 2-8°C resulting in a slight opaque white liquid. At room temperature liquid is clear and colorless. |
| Applications: | FC, ICC |
| Recommended Usage: | FC |
| Reactivity: | Human |
| Host Species: | Mouse |
| Specificity: | Clone B7/21 recognizes a monomorphic epitope on human HLA-DP1, -DP2, -DP3, -DP4, and -DP5. It does not cross-react with HLA-DR or HLA-DQ. |
| Antigen Distribution: | HLA-DP is expressed on antigen-presenting cells, including macrophages, monocytes, DCs, and B cells, and activated T cells. |
| Immunogen: | Unknown |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. Note: B7/21 is an antibody prone to precipitation at 2-8°C resulting in a slight opaque white liquid. At room temperature liquid is clear and colorless. |
HLA-DP antibody, clone B7/21, recognizes the major histocompatibility complex (MHC) class II molecule Human Leukocyte Antigen - DP isotype (HLA-DP). MHC class II is constitutively expressed on human professional antigen-presenting cells (APCs), including macrophages/monocytes, dendritic cells (DCs), and B cells, and is induced on T cells upon activation1. HLA-DP consists of two transmembrane proteins, a 35 kDa α (heavy) chain and 29 kDa β (light) chain2 encoded by the HLA-DPA1 and HLA-DPB1 genes, respectively, located in the HLA complex of chromosome 6. The N-terminal α1 and β1 domains form the antigen-binding groove, which binds 13-25 aa peptides derived from exogenous antigens3. On APCs, MHC class II plays a critical role in the adaptive immune response by presenting phagocytosed antigens to helper CD4 T cells. The T cell receptor (TCR)/CD3 complex of CD4 T cells interacts with peptide-MHC class II, which induces CD4 T cell activation leading to the coordination and regulation of other effector cells. CD4 molecules also bind to MHC class II, which helps augment TCR signaling4. It has also been demonstrated that MHC class II express on activated T cells are capable of antigen presentation5 and can transduce signals into T cells, enhancing T cell proliferation and activity6. High HLA-DP expression is associated with an increased risk of graft-versus-host disease7. Specific alleles of HLA-DP are associated with autoimmune diseases, including rheumatoid arthritis8.
| Technical Datasheet: | View |
| Protein: | HLA-DP Monomorphic |
| Research Area: | Immunology, Innate Immunity |
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