Anti-Human HLA A2,B7 (MHC Class I) In Vivo Antibody - Low Endotoxin
- SKU:
- IVMB0130
- Product Type:
- In Vivo Monoclonal Antibody
- Clone:
- BB7.6
- Protein:
- HLA-A2,B7 ⋅ MHC Class I
- Isotype:
- Mouse IgG1
- Reactivity:
- Human
- Synonyms:
- HLA-A
- HLA-A2,B7
- B.C.HLA-A2
- Endotoxin Level:
- Low Endotoxin
- Host Species:
- Mouse
- Applications:
- FC
Description
| Product Name: | Anti-Human HLA A2,B7 (MHC Class I) In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0130 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | BB7.6 |
| Protein: | HLA-A2,B7 ⋅ MHC Class I |
| Product Type: | Monoclonal Antibody |
| Synonyms: | HLA-A, HLA-A2,B7, B.C.HLA-A2 |
| Isotype: | Mouse IgG1 |
| Reactivity: | Human |
| Immunogen: | Solubilized HLA-B7 antigen |
| Applications: | FC |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | FC |
| Reactivity: | Human |
| Host Species: | Mouse |
| Specificity: | Clone BB7.6 recognizes an epitope shared by the human MHC class I molecule HLA-B. |
| Antigen Distribution: | HLA-B is ubiquitously expressed on nucleated cells. |
| Immunogen: | Solubilized HLA-B7 antigen |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
HLA-B antibody, clone BB7.6, recognizes a shared epitope (Bw6) of the major histocompatibility complex (MHC) class I molecule human leukocyte antigen (HLA)-B1. MHC class I is ubiquitously expressed on the cell surface of nucleated cells and consists of a 45-kDa type I transmembrane glycoprotein (α-chain or heavy chain) and a 12-kDa soluble protein (β2-microglobulin, β2M)2,3. The α-chain consists of three domains (α1, α2, and α3)4. α1 and α2 form the closed antigen-binding groove and bind to 8-10 aa peptides derived from cytosolic antigens5-7. β2M noncovalently associates with α3, which is essential for MHC stability. MHC class I plays a critical role in the adaptive immune response by presenting endogenous antigens to cytotoxic CD8 T cells. MHC class I molecules can also present exogenous antigens to CD8 T cells via a process known as cross-presentation8. The T cell receptor (TCR)/CD3 complex of CD8 T cells interacts with peptide-MHC class I, which induces CD8 T cell activation and subsequent cell-killing. CD8 molecules also bind to MHC class I, which helps augment TCR signaling9. In contrast to CD8 T cells, MHC class I is an inhibitory ligand for natural killer (NK) cells, promoting self tolerance10. MHC class I also contributes to the positive selection of CD8 T cells and NK cell specificity11,12.
| Technical Datasheet: | View |
| Protein: | HLA-A2,B7 ⋅ MHC Class I |
| Research Area: | Immunology |
| Mouse IgG1 Isotype Control [HKSP] | |
|---|---|
| Clone | HKSP |
| Isotype | Mouse IgG1 |
| Endotoxin Level | Low Endotoxin |
| Mouse IgG1 Isotype Control [HKSP84] | |
|---|---|
| Clone | HKSP84 |
| Isotype | Mouse IgG1 |
| Endotoxin Level | Low Endotoxin |
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