Anti-Human HLA-A, B, C (MHC Class I) In Vivo Antibody - Low Endotoxin
- SKU:
- IVMB0014
- Product Type:
- In Vivo Monoclonal Antibody
- Clone:
- W6/32
- Protein:
- HLA-A,B,C
- Isotype:
- Mouse IgG2a kappa
- Reactivity:
- Baboon
- Chimpanzee
- Cynomolgus Monkey
- Feline
- Bovine
- Human
- Synonyms:
- Major Histocompatibility Class I
- MHC class I
- human leukocyte antigen (HLA)
- Endotoxin Level:
- Low Endotoxin
- Host Species:
- Mouse
- Applications:
- Blocking
- CODEX
- FC
- IHC FF
- IP
- WB
Description
| Product Name: | Anti-Human HLA-A, B, C (MHC Class I) In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0014 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | W6/32 |
| Protein: | HLA-A,B,C |
| Product Type: | Monoclonal Antibody |
| Synonyms: | Major Histocompatibility Class I, MHC class I, human leukocyte antigen (HLA) |
| Isotype: | Mouse IgG2a κ |
| Reactivity: | Baboon, Chimpanzee, Cynomolgus Monkey, Feline, Bovine, Human |
| Immunogen: | Human tonsil cell membrane |
| Applications: | B, FC, IHC FF, IP, PhenoCycler®, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | B, CODEX®, FC, IHC FF, IP, WB |
| Recommended Usage: | FC The suggested concentration for this HLA-A,B,C Clone W6/32 antibody for staining cells in flow cytometry is ≤ 2.0 µg per 106 in a volume of 100 µl or 100µl of whole blood. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for for use in western blotting is 1-10 µg/ml. |
| Reactivity: | Baboon, Chimpanzee, Cynomolgus Monkey, Feline, Bovine, Human |
| Host Species: | Mouse |
| Specificity: | Clone W6/32 recognizes the human MHC class I molecules HLA-A, -B, and -C. |
| Antigen Distribution: | HLA-A, -B, and -C are ubiquitously expressed on nucleated cells. |
| Immunogen: | Human tonsil cell membrane |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. |
HLA antibody, clone W6/32, recognizes the major histocompatibility complex (MHC) class I molecules human leukocyte antigen (HLA)-A, HLA-B, and HLA-C. MHC class I is ubiquitously expressed on the cell surface of nucleated cells and consists of a 45-kDa type I transmembrane glycoprotein (α-chain or heavy chain) and a 12-kDa soluble protein (β2-microglobulin, β2M)1,2. The α-chain consists of three domains (α1, α2, and α3)3. α1 and α2 form the closed antigen-binding groove and bind to 8-10 aa peptides derived from cytosolic antigens4-6. β2M noncovalently associates with α3, which is essential for MHC stability. MHC class I plays a critical role in the adaptive immune response by presenting endogenous antigens to cytotoxic CD8 T cells. MHC class I molecules can also present exogenous antigens to CD8 T cells via a process known as cross-presentation7. The T cell receptor (TCR)/CD3 complex of CD8 T cells interacts with peptide-MHC class I, which induces CD8 T cell activation and subsequent cell-killing. CD8 molecules also bind to MHC class I, which helps augment TCR signaling8. In contrast to CD8 T cells, MHC class I is an inhibitory ligand for natural killer (NK) cells, promoting self tolerance9. MHC class I also contributes to the positive selection of CD8 T cells and NK cell specificity10,11.
| Technical Datasheet: | View |
| Protein: | HLA-A,B,C |
| Function: | Antigen presentation |
| Ligand/Receptor: | CD3/TCR, CD8 |
| Research Area: | Immunology, Innate Immunity |
| Mouse IgG2a Isotype Control | |
|---|---|
| Clone | C1.18.4 |
| Isotype | Mouse IgG2a |
| Endotoxin Level | Low Endotoxin |
Meet the team!
Shane Costigan
Territory Manager & Team Lead
Abdul Khadim
Sales Executive
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