Anti-Human CD8 In Vivo Antibody - Ultra Low Endotoxin
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- SKU:
- IVMB0125
- Product Type:
- In Vivo Monoclonal Antibody
- Clone:
- UCHT-4
- Protein:
- CD8
- Isotype:
- Mouse IgG2a
- Reactivity:
- Human
- Endotoxin Level:
- Ultra Low Endotoxin
- Host Species:
- Mouse
- Applications:
- FA
- FC
- WB
Frequently bought together:
Description
system_update_altTechnical Datasheet
| Product Name: | Anti-Human CD8 In Vivo Antibody - Ultra Low Endotoxin |
| Product Code: | IVMB0125 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | UCHT-4 |
| Protein: | CD8 |
| Product Type: | Monoclonal Antibody |
| Isotype: | Mouse IgG2a |
| Reactivity: | Human |
| Immunogen: | Thymocytes and Sézary T cells |
| Applications: | FA, FC, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | <0.5 EU/mg as determined by the LAL method |
| Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | FA, FC, WB |
| Recommended Usage: | FC The suggested concentration for this UCHT-4 antibody for staining cells in flow cytometry is ≤ 1.0 µg per 106 in a volume of 100 µl or 100µl of whole blood. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for this UCHT-4 antibody for use in western blotting is 1-10 µg/ml when used in conjunction with compatible secondary reagents, under either reducing or non-reducing conditions. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. |
| Reactivity: | Human |
| Host Species: | Mouse |
| Specificity: | Clone UCHT-4 recognizes human CD8. |
| Antigen Distribution: | CD8 is expressed on blood lymphocytes, a subset of NK cells, and thymocytes. Persons with HIV exhibit increased levels of CD8+ lymphocytes. |
| Immunogen: | Thymocytes and Sézary T cells |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | <0.5 EU/mg as determined by the LAL method |
| Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
CD8 is made up of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8 is part of the Ig superfamily that expresses primarily as CD8a homodimers. CD8a is a 32-34 kD type I glycoprotein that can also form heterodimers with CD8b. CD8 is an antigen co-receptor on T cells that mediates efficient cell to cell interactions within the immune system. CD8 coupled with the T cell receptor on the T lymphocyte recognizes an antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The CD8 co-receptor also plays a role in T cell signaling by interacting with Lck (lymphocyte-specific protein tyrosine kinase) which leads to the activation of transcription factors that affect the expression of certain genes.
| Technical Datasheet: | View |
| Protein: | CD8 |
| Ligand/Receptor: | MHC Class I molecules |
| Research Area: | Immunology |
| Mouse IgG2a Isotype Control | |
|---|---|
| Clone | C1.18.4 |
| Isotype | Mouse IgG2a kappa |
| Endotoxin Level | Ultra Low Endotoxin |
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