Anti-Human CD45RO [UCHL-1] In Vivo Antibody - Low Endotoxin

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SKU:
IVMB0311
Product Type:
In Vivo Monoclonal Antibody
Clone:
UCHL-1
Protein:
CD45RO
Isotype:
IgG2a
Reactivity:
Human
Synonyms:
B220
Research Area:
Cell Biology
Immunology
Inhibitory Molecules
Neuroscience
Neuroscience Cell Markers
Stem Cell
Endotoxin Level:
Low Endotoxin
Host Species:
Mouse
Applications:
FC
IHC FF
IHC FFPE
In Vivo
IP
PhenoCycler
WB
Frequently bought together:

Description

Product Name:Anti-Human CD45RO (Clone UCHL-1) In Vivo Antibody - Low Endotoxin
Product Code:IVMB0311
Size:1 mg, 5 mg, 25 mg, 50 mg, 100 mg
Clone:UCHL-1
Protein:CD45RO
Product Type:Monoclonal Antibody
Synonyms:B220
Isotype:IgG2a
Reactivity:Human
Applications:FC, IHC FF, IHC FFPE, In Vivo, IP, PhenoCycler®, WB
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Endotoxin Level:< 1.0 EU/mg as determined by the LAL method
Purity:≥95% monomer by analytical SEC ⋅ >95% by SDS Page
Product Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Applications:FC, IHC FF, IHC FFPE, In Vivo, IP, PhenoCycler®, WB
Reactivity:Human
Host Species:Mouse
Specificity:Clone UCHL-1 recognizes human CD45RO.
Antigen Distribution:The CD45RO antigen is present on most thymocytes, about 40% of resting peripheral blood T-lymphocytes and the majority of T-cells in skin reactive infiltrates and T-cell malignancies. CD45RO is also found on a subset of B-cells and some B-cell lymphomas. NK cells do not express the CD45RO antigen. It is also present on monocytes, macrophages and granulocytes.
Concentration:≥ 5.0 mg/ml
Endotoxin Level:< 1.0 EU/mg as determined by the LAL method
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Purity:≥95% monomer by analytical SEC ⋅ >95% by SDS Page
Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

CD45 is a 180-240kD glycoprotein member of the protein tyrosine phosphatase (PTP) family known for its involvement in regulating a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45 and its isoforms are vital regulators of T- and B-cell antigen receptor signaling. CD45 functions through its extracellular domain or through its cytoplasmic domain, and serves as a negative regulator of cytokine receptor signaling via JAK kinase supression. The large extracellular domain is highly glycosylated, and its multiple isoforms allow extensive variation in the structure of its side chains. CD45 isoforms show cell-type and differentiation-stage specific expression that can be used as markers that identify and distinguish between different types of immune cells. CD45R is an isoform of CD45 with a molecular weight of 220 kD. CD45R contains all three possible exons (A, B, and C); making it the longest protein generated from alternative splicing with a migration at 200 kD when isolated from T cells. Furthermore, B cells express CD45R with heavier glycosylation, bringing the molecular weight to 220 kD, hence the name B220. Notably, B220 expression is not only restricted to B cells and may also be expressed on activated T cells, on a subset of dendritic cells, and on other antigen-presenting cells. Additionally, activated and memory T lymphocytes express CD45RO which facilitates T cell activation. CD45RO lacks all three possible exons (A, B, and C), making it the shortest CD45 isoform.

Protein:CD45RO
Ligand/Receptor:CD22
Research Area:Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers, Stem Cell
Shipping:2-8°C
PubMed:CD45RO
NCBI Gene Bank ID:5788
UniProt:CD45R

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