Anti-Human CD25 (IL-2Rα) [7G7B6] In Vivo Antibody - Low Endotoxin

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SKU:
IVMB0011
Product Type:
In Vivo Monoclonal Antibody
Clone:
7G7B6
Protein:
CD25
Isotype:
Mouse IgG2a
Reactivity:
Human
Synonyms:
IL-2Ralpha
CD25
Ly-43
p55
Tac
Endotoxin Level:
Low Endotoxin
Host Species:
Mouse
Applications:
ELISA
FC
IF Staining
IP
WB
Frequently bought together:

Description

system_update_altTechnical Datasheet
Product Name:Anti-Human CD25 (IL-2Rα) [7G7B6] In Vivo Antibody - Low Endotoxin
Product Code:IVMB0011
Size:1mg, 5mg, 25mg, 50mg, 100mg
Clone:7G7B6
Protein:CD25
Product Type:Monoclonal Antibody
Synonyms:IL-2Rα, CD25, Ly-43, p55, Tac
Isotype:Mouse IgG2a
Reactivity:Human
Immunogen:Recombinant human peripheral blood mononuclear cells (PBMCs)
Applications:ELISA, FC, IF Staining, IP, WB
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:< 1.0 EU/mg as determined by the LAL method
Purity:≥95% monomer by analytical SEC
>95% by SDS Page
Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Applications:ELISA, FC, IF Staining, IP, WB
Recommended Usage:ELISA
FC
WB
Reactivity:Human
Host Species:Mouse
Specificity:Clone 7G7B6 reacts with human IL-2Rα.
Antigen Distribution:IL-2Rα is expressed on activated mature T and B lymphocytes, during early stages of thymocytes development, pre-B cells, and in activated CD4+ memory T-lymphocytes.
Immunogen:Recombinant human peripheral blood mononuclear cells (PBMCs)
Concentration:≥ 5.0 mg/ml
Endotoxin Level:< 1.0 EU/mg as determined by the LAL method
Purity:≥95% monomer by analytical SEC
>95% by SDS Page
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

CD25 (IL-2Rα) is the 55 kDa subunit of the interleukin 2 receptor alpha chain which has been shown to play roles in lymphocyte differentiation, activation, and proliferation. IL-2Rα is a transmembrane glycoprotein comprised of a heavily glycosylated extracellular domain, a transmembrane region and a short cytoplasmic tail. IL-2Rα heterodimerizes with IL-2Rβ (CD122) and IL-2Rγ (CD132) and forms the IL-2 receptor complex. IL-2Rα by itself binds IL-2 with relatively low affinity, while the IL-2 receptor complex binds IL-2 with high affinity. IL-2Rα mutations cause severe immunodeficiency.

Technical Datasheet:View
Protein:CD25
Research Area:Immunology
Product Thumbnail
Mouse IgG2a Isotype Control
Clone C1.18.4
Isotype Mouse IgG2a
Endotoxin Level Low Endotoxin
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