Anti-Human CD14 In Vivo Antibody - Low Endotoxin
- SKU:
- IVMB0111
- Product Type:
- In Vivo Monoclonal Antibody
- Clone:
- UCHM-1
- Protein:
- CD14
- Isotype:
- Mouse IgG2a
- Reactivity:
- Human
- Synonyms:
- P35
- Endotoxin Level:
- Low Endotoxin
- Host Species:
- Mouse
- Applications:
- FC
- WB
Frequently bought together:
Description
system_update_altTechnical Datasheet
| Product Name: | Anti-Human CD14 In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0111 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | UCHM-1 |
| Protein: | CD14 |
| Product Type: | Monoclonal Antibody |
| Synonyms: | P35 |
| Isotype: | Mouse IgG2a |
| Reactivity: | Human |
| Applications: | FC, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | FC, WB |
| Recommended Usage: | FC The suggested concentration for this UCHM-1 antibody for staining cells in flow cytometry is ≤ 1.0 µg per 106 in a volume of 100 µl or 100µl of whole blood. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for this UCHM-1 antibody for use in western blotting is 1-10 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. |
| Reactivity: | Human |
| Host Species: | Mouse |
| Specificity: | Clone UCHM-1 recognizes an epitope on human CD14. |
| Antigen Distribution: | CD14 is expressed on monocytes, macrophages, dendritic cells, and granulocytes. |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
CD14 is a 53-55 kD GPI-anchored single chain glycoprotein that plays a role in the clearance of gram-negative pathogens by interacting with other proteins, including TLR-4 and MD-2, to mediate the innate immune response to bacterial lipopolysaccharide (LPS). As a high-affinity receptor for LPS, it is involved in the upregulation of adhesion molecules and cytokine expression in monocytes and neutrophils.
| Technical Datasheet: | View |
| Protein: | CD14 |
| Ligand/Receptor: | LPS |
| Research Area: | Cell Biology, Immunology, Neuroinflammation, Neuroscience |
| Mouse IgG2a Isotype Control | |
|---|---|
| Clone | C1.18.4 |
| Isotype | Mouse IgG2a |
| Endotoxin Level | Low Endotoxin |
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