Anti-Human CD11c In Vivo Antibody - Ultra Low Endotoxin
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- SKU:
- IVMB0110
- Product Type:
- In Vivo Monoclonal Antibody
- Clone:
- 3.9
- Protein:
- CD11c
- Isotype:
- Mouse IgG1 kappa
- Reactivity:
- Human
- Synonyms:
- Integrin alphaX subunit
- CR4
- p150
- ITGAX
- Research Area:
- Adhesion & Migration
- Endotoxin Level:
- Ultra Low Endotoxin
- Host Species:
- Mouse
- Applications:
- Blocking
- CyTOF
- FA
- FC
- IHC FF
- WB
Frequently bought together:
Description
system_update_altTechnical Datasheet
| Product Name: | Anti-Human CD11c In Vivo Antibody - Ultra Low Endotoxin |
| Product Code: | IVMB0110 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | 3.9 |
| Protein: | CD11c |
| Product Type: | Monoclonal Antibody |
| Synonyms: | Integrin αX subunit, CR4, p150, ITGAX |
| Isotype: | Mouse IgG1 κ |
| Reactivity: | Human |
| Immunogen: | Rheumatoid synovial fluid cells and fibronectin purified human monocytes |
| Applications: | B, CyTOF®, FA, FC, IHC FF, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | <0.5 EU/mg as determined by the LAL method |
| Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | B, CyTOF®, FA, FC, IHC FF, WB |
| Recommended Usage: | FC The suggested concentration for clone 3.9 antibody for staining cells in flow cytometry is ≤ 2.0 µg per 106 in a volume of 100 µl or 100µl of whole blood. Titration of the reagent is recommended for optimal performance for each application.> WB The suggested concentration for this 3.9 antibody for use in western blotting is 1-10 µg/ml. A suggested positive control for Western blotting is a Mouse Skeletal Muscle Tissue Lysate. |
| Reactivity: | Human |
| Host Species: | Mouse |
| Specificity: | Clone 3.9 recognizes the α-chain (CD11c) of the CD11c/CD18 complex. It is specific for the I domain of CD11c. Clone 3.9 binds the activated form of CD11c and partially blocks the binding of CD11c with ICAM-4. |
| Antigen Distribution: | CD11c is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. |
| Immunogen: | Rheumatoid synovial fluid cells and fibronectin purified human monocytes |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | <0.5 EU/mg as determined by the LAL method |
| Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
LFA-1α (CD11a) and CD18 are the Integrin alpha-L and beta-2 chains respectively that combine to form LFA-1, a glycoprotein and a member of the Integrin family. Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3, ICAM4 and for F11R. LFA-1 participates in the immunological synapses between CD8+ T lymphocytes and antigen-presenting cells. The absence of LFA-1α or β may induce LAD. The antigen contributes to natural killer cell cytotoxicity, and is involved in various immune phenomena such as leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. The CD11b/CD18 antigen is a heterodimeric surface glycoprotein on leukocytes and belongs to the β2 integrin family. CD11b functions as a receptor for C3bi complement, clotting factor X, fibrinogen and ICAM-1. CD11c forms an α/β heterodimeric glycoprotein (CD11c/CD18 complex) which belongs to the β2 integrin family. The complex binds fibrinogen and reportedly serves as a receptor for iC3b and ICAM-1. During inflammatory responses, it mediates cell to cell interaction and is important in both monocyte adhesion and chemotaxis.
| Technical Datasheet: | View |
| Protein: | CD11c |
| Function: | Adhesion, CTL killing |
| Ligand/Receptor: | CD54, fibrinogen, iC3b, ICAM-1, ICAM-4 |
| Research Area: | Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers |
| Mouse IgG1 Isotype Control [HKSP84] | |
|---|---|
| Clone | HKSP84 |
| Isotype | Mouse IgG1 |
| Endotoxin Level | Ultra Low Endotoxin |
| Mouse IgG1 Isotype Control [HKSP] | |
|---|---|
| Clone | HKSP |
| Isotype | Mouse IgG1 |
| Endotoxin Level | Ultra Low Endotoxin |
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