Anti-HTRA2 Antibody (CAB14877)
- SKU:
- CAB14877
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Frequently bought together:
Description
| Antibody Name: | Anti-HTRA2 Antibody |
| Antibody SKU: | CAB14877 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 334-458 of human HTRA2 (NP_037379.1). |
| Application: | WB IHC IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SW480, HT-29, MCF7, HL-60 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 334-458 of human HTRA2 (NP_037379.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PSDR LREF LHRG EKKN SSSG ISGS QRRY IGVM MLTL SPSI LAEL QLRE PSFP DVQH GVLI HKVI LGSP AHRA GLRP GDVI LAIG EQMV QNAE DVYE AVRT QSQL AVQI RRGR ETLT LYVT PEVT E |
| Gene ID: | 27429 |
| Uniprot: | O43464 |
| Cellular Location: | Mitochondrion intermembrane space, Mitochondrion membrane, Single-pass membrane protein |
| Calculated MW: | 38kDa/39kDa/46kDa/48kDa |
| Observed MW: | 36kDa |
| Synonyms: | HTRA2, MGCA8, OMI, PARK13, PRSS25 |
| Background: | This gene encodes a serine protease. The protein has been localized in the endoplasmic reticulum and interacts with an alternatively spliced form of mitogen-activated protein kinase 14. The protein has also been localized to the mitochondria with release to the cytosol following apoptotic stimulus. The protein is thought to induce apoptosis by binding the apoptosis inhibitory protein baculoviral IAP repeat-containing 4. Nuclear localization of this protein has also been observed. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. |
| UniProt Protein Function: | HTRA2: a serine proteinase that normally resides in mitochondrial membranes. It exists in two populations in mitochondria: as an unprocessed form probably attached to the inner mitochondrial membrane through a N-terminal transmembrane domain, and as a processed form containing a reaper motif at its N-terminus. Following apoptotic stimuli it is autoproteolytically activated and released into the cytosol, where it promotes programmed cell death in caspase-dependent and -independent manners. The amino-terminal reaper motif binds to the IAP (inhibitor of apoptosis) proteins cIAP1, cIAP2, and and XIAP, disrupting IAP-caspase complexes in a manner similar to Smac/DIABLO. Phosphorylation by the protein kinase Akt attenuates its serine protease and pro-apoptotic activities. The PDZ domain mediates interaction with Mxi2, an alternatively spliced form of the p38 stress-activated kinase. In contrast to its pro-apoptotic effects, targeted deletion in mice indicates that it is involved in protection against cell stress in striatial neurons. Defects in HTRA2 are the cause of Parkinson disease 13 (PARK13). Four alternatively spliced human isoforms have been described. |
| UniProt Protein Details: | Protein type:Protease; Mitochondrial; Membrane protein, integral; EC 3.4.21.108 Chromosomal Location of Human Ortholog: 2p12 Cellular Component: chromatin; cytoskeleton; cytosol; endoplasmic reticulum membrane; internal side of plasma membrane; membrane; mitochondrial intermembrane space; mitochondrion; nucleus Molecular Function:peptidase activity; protein binding; serine-type endopeptidase activity; serine-type peptidase activity Biological Process: cellular protein catabolic process; DNA damage response, signal transduction resulting in induction of apoptosis; negative regulation of cell cycle; positive regulation of apoptosis; positive regulation of caspase activity; protein autoprocessing; proteolysis Disease: Parkinson Disease 13, Autosomal Dominant, Susceptibility To |
| NCBI Summary: | This gene encodes a serine protease. The protein has been localized in the endoplasmic reticulum and interacts with an alternatively spliced form of mitogen-activated protein kinase 14. The protein has also been localized to the mitochondria with release to the cytosol following apoptotic stimulus. The protein is thought to induce apoptosis by binding the apoptosis inhibitory protein baculoviral IAP repeat-containing 4. Nuclear localization of this protein has also been observed. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016] |
| UniProt Code: | O43464 |
| NCBI GenInfo Identifier: | 17376879 |
| NCBI Gene ID: | 27429 |
| NCBI Accession: | O43464.2 |
| UniProt Secondary Accession: | O43464,Q9HBZ4, Q9P0Y3, Q9P0Y4, |
| UniProt Related Accession: | O43464 |
| Molecular Weight: | 39,914 Da |
| NCBI Full Name: | Serine protease HTRA2, mitochondrial |
| NCBI Synonym Full Names: | HtrA serine peptidase 2 |
| NCBI Official Symbol: | HTRA2 |
| NCBI Official Synonym Symbols: | OMI; PARK13; PRSS25 |
| NCBI Protein Information: | serine protease HTRA2, mitochondrial |
| UniProt Protein Name: | Serine protease HTRA2, mitochondrial |
| UniProt Synonym Protein Names: | High temperature requirement protein A2; HtrA2; Omi stress-regulated endoprotease; Serine protease 25; Serine proteinase OMI |
| Protein Family: | Serine protease |
| UniProt Gene Name: | HTRA2 |
| UniProt Entry Name: | HTRA2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using HTRA2 Rabbit pAb (CAB14877) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). |
 | Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug HTRA2 antibody (CAB14877). Western blot was performed from the immunoprecipitate using HTRA2 antibody (CAB14877) at a dilition of 1:1000. |
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