Anti-HSPB8 Antibody (CAB2514)
- SKU:
- CAB2514
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-HSPB8 Antibody |
| Antibody SKU: | CAB2514 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-196 of human HSPB8 (NP_055180.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | RD, Mouse liver, Mouse heart, Rat heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-196 of human HSPB8 (NP_055180.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MADG QMPF SCHY PSRL RRDP FRDS PLSS RLLD DGFG MDPF PDDL TASW PDWA LPRL SSAW PGTL RSGM VPRG PTAT ARFG VPAE GRTP PPFP GEPW KVCV NVHS FKPE ELMV KTKD GYVE VSGK HEEK QQEG GIVS KNFT KKIQ LPAE VDPV TVFA SLSP EGLL IIEA PQVP PYST FGES SFNN ELPQ DSQE VTCT |
| Gene ID: | 26353 |
| Uniprot: | Q9UJY1 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 21kDa |
| Observed MW: | 22KDa |
| Synonyms: | HSPB8, CMT2L, DHMN2, E2IG1, H11, HMN2, HMN2A, HSP22 |
| Background: | The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease. |
| UniProt Protein Function: | HSPB8: Displays temperature-dependent chaperone activity. Monomer. Interacts with HSPB1. Interacts with DNAJB6. By 17-beta-estradiol. Predominantly expressed in skeletal muscle and heart. Belongs to the small heat shock protein (HSP20) family. |
| UniProt Protein Details: | Protein type:Heat shock protein Chromosomal Location of Human Ortholog: 12q24.23 Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; intracellular; nucleus Molecular Function:identical protein binding; protein binding; protein kinase activity Disease: Charcot-marie-tooth Disease, Axonal, Type 2l; Neuronopathy, Distal Hereditary Motor, Type Iia |
| NCBI Summary: | The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9UJY1 |
| NCBI GenInfo Identifier: | 13431576 |
| NCBI Gene ID: | 26353 |
| NCBI Accession: | Q9UJY1.1 |
| UniProt Secondary Accession: | Q9UJY1,Q6FIH3, Q9UKS3, B2R6A6, |
| UniProt Related Accession: | Q9UJY1 |
| Molecular Weight: | 196 |
| NCBI Full Name: | Heat shock protein beta-8 |
| NCBI Synonym Full Names: | heat shock 22kDa protein 8 |
| NCBI Official Symbol: | HSPB8 |
| NCBI Official Synonym Symbols: | H11; HMN2; CMT2L; DHMN2; E2IG1; HMN2A; HSP22 |
| NCBI Protein Information: | heat shock protein beta-8; protein kinase H11; alpha-crystallin C chain; E2-induced gene 1 protein; heat shock 27kDa protein 8; small stress protein-like protein HSP22 |
| UniProt Protein Name: | Heat shock protein beta-8 |
| UniProt Synonym Protein Names: | Alpha-crystallin C chain; E2-induced gene 1 protein; Protein kinase H11; Small stress protein-like protein HSP22 |
| Protein Family: | Heat shock protein |
| UniProt Gene Name: | HSPB8 |
| UniProt Entry Name: | HSPB8_HUMAN |
 | Western blot analysis of extracts of various cell lines, using HSPB8 antibody (CAB2514) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded rat heart using HSPB8 Rabbit pAb (CAB2514) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using HSPB8 antibody (CAB2514). Blue: DAPI for nuclear staining. |
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