Anti-HSP70 Antibody (CAB12948)

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SKU:
CAB12948
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
Frequently bought together:

Description

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Antibody Name:Anti-HSP70 Antibody
Antibody SKU:CAB12948
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 500-600 of human HSP70 (NP_005336.3).
Application:WB IHC IF
Recommended Dilution:WB 1:1000 - 1:3000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, HepG2, BxPC-3, 293T, A-549, Mouse brain, Mouse kidney, Mouse lung, Rat lung
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 500-600 of human HSP70 (NP_005336.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:KITI TNDK GRLS KEEI ERMV QEAE KYKA EDEV QRER VSAK NALE SYAF NMKS AVED EGLK GKIS EADK KKVL DKCQ EVIS WLDA NTLA EKDE FEHK RKEL E
Gene ID:3303
Uniprot:P0DMV8
Cellular Location:
Calculated MW:70kDa
Observed MW:70kDa
Synonyms:HSPA1A, HEL-S-103, HSP70-1, HSP70-1A, HSP70.1, HSP70I, HSP72, HSPA1, HSP70
Background:This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.
UniProt Protein Function:HSP70: a critical chaperone protein that has a high affinity for unfolded polypeptide chains. It binds extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In cooperation with other chaperones, hsp70 stabilizes preexistent proteins against aggregation and mediates the folding of newly translated polypeptides in the cytosol as well as within organelles. Mitochondrial HSP70 is crucial to the import process: mutant forms of HSP70 fail to import precursor proteins. Is anti-apoptotic in sympathetic neurones and mediates this effect primarily by suppressing c-Jun transcriptional signalling. Interacts with tau protein and mediates proper folding of tau. Can promote the degradation of tau protein. Triptolide, a potential therapeutic agent for progression/metastasis of pancreatic cancer, causes pancreatic cancer cell death by induction of apoptosis, an effect mediated by the inhibition of HSP70.
UniProt Protein Details:

Protein type:Chaperone; Heat shock protein; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6p21.33

Cellular Component: centriole; centrosome; cytoplasm; cytosol; endoplasmic reticulum; extracellular region; focal adhesion; inclusion body; mitochondrion; nuclear speck; nucleoplasm; nucleus; perinuclear region of cytoplasm; ribonucleoprotein complex; ubiquitin ligase complex; vesicle

Molecular Function:ATP binding; ATPase activity; ATPase activity, coupled; cadherin binding; denatured protein binding; enzyme binding; G-protein-coupled receptor binding; heat shock protein binding; histone deacetylase binding; protein binding; protein N-terminus binding; receptor binding; RNA binding; ubiquitin protein ligase binding; unfolded protein binding

Biological Process: activation of NF-kappaB transcription factor; ATP metabolic process; chaperone-mediated protein complex assembly; mRNA catabolic process; negative regulation of apoptosis; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of protein ubiquitination; negative regulation of transforming growth factor beta receptor signaling pathway; neutrophil degranulation; positive regulation of interleukin-8 production; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein refolding; protein stabilization; regulation of mRNA stability; regulation of protein ubiquitination; response to unfolded protein

NCBI Summary:This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008]
UniProt Code:P0DMV8
NCBI GenInfo Identifier:825168577
NCBI Gene ID:3303
NCBI Accession:P0DMV8.1
UniProt Secondary Accession:P0DMV8,P08107, P19790, Q5JQI4, Q5SP17, Q9UQL9, Q9UQM0 B4E3B6,
UniProt Related Accession:P0DMV8
Molecular Weight:70kDa
NCBI Full Name:Heat shock 70 kDa protein 1A
NCBI Synonym Full Names:heat shock protein family A (Hsp70) member 1A
NCBI Official Symbol:HSPA1A  
NCBI Official Synonym Symbols:HSP72; HSPA1; HSP70I; HSP70-1; HSP70.1; HSP70-1A; HEL-S-103  
NCBI Protein Information:heat shock 70 kDa protein 1A
UniProt Protein Name:Heat shock 70 kDa protein 1A
UniProt Synonym Protein Names:Heat shock 70 kDa protein 1; HSP70-1
Protein Family:Heat shock 70 kDa protein
UniProt Gene Name:HSPA1A  
Anti-HSP70 Antibody (CAB12948)Western blot analysis of extracts of various cell lines, using HSP70 antibody (CAB12948) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-HSP70 Antibody (CAB12948)Immunohistochemistry of paraffin-embedded human lung cancer using HSP70 antibody (CAB12948) at dilution of 1:100 (40x lens).
Anti-HSP70 Antibody (CAB12948)Immunohistochemistry of paraffin-embedded mouse testis using HSP70 antibody (CAB12948) at dilution of 1:100 (40x lens).
Anti-HSP70 Antibody (CAB12948)Immunohistochemistry of paraffin-embedded rat testis using HSP70 antibody (CAB12948) at dilution of 1:100 (40x lens).
Anti-HSP70 Antibody (CAB12948)Immunofluorescence analysis of C6 cells using HSP70 Rabbit pAb (CAB12948) at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.

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