Anti-HSD17B12 항체 (CAB17151)

Antibody Name:	Anti-HSD17B12 Antibody
Antibody SKU:	CAB17151
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 43-312 of human HSD17B12 (NP_057226.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 43-312 of human HSD17B12 (NP_057226.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AGVG PGLG EWAV VTGS TDGI GKSY AEEL AKHG MKVV LISR SKDK LDQV SSEI KEKF KVET RTIA VDFA SEDI YDKI KTGL AGLE IGIL VNNV GMSY EYPE YFLD VPDL DNVI KKMI NINI LSVC KMTQ LVLP GMVE RSKG AILN ISSG SGML PVPL LTIY SATK TFVD FFSQ CLHE EYRS KGVF VQSV LPYF VATK LAKI RKPT LDKP SPET FVKS AIKT VGLQ SRTN GYLI HALM GSII SNLP SWIY LKIV MNMN KSTR AHYL KKTK KN
Gene ID:	51144
Uniprot:	Q53GQ0
Cellular Location:
Calculated MW:
Observed MW:	Refer to figures

Synonyms:	HSD17B12, KAR, SDR12C1
Background:	This gene encodes a very important 17beta-hydroxysteroid dehydrogenase (17beta-HSD) that converts estrone into estradiol in ovarian tissue. This enzyme is also involved in fatty acid elongation.

UniProt Protein Function:	HSD17B12: Catalyzes the transformation of estrone (E1) into estradiol (E2), suggesting a central role in estrogen formation. Its strong expression in ovary and mammary gland suggest that it may constitute the major enzyme responsible for the conversion of E1 to E2 in women. Also has 3-ketoacyl-CoA reductase activity, reducing both long chain 3-ketoacyl-CoAs and long chain fatty acyl-CoAs, suggesting a role in long fatty acid elongation. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.
UniProt Protein Details:	Protein type:Membrane protein, integral; Oxidoreductase; Lipid Metabolism - androgen and estrogen; EC 1.1.1.62; Lipid Metabolism - unsaturated fatty acid biosynthesis; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 11p11.2 Cellular Component: extracellular matrix; endoplasmic reticulum membrane; integral to membrane Molecular Function:heparin binding; collagen binding; estradiol 17-beta-dehydrogenase activity; protein binding; fibronectin binding Biological Process: extracellular matrix organization and biogenesis; triacylglycerol biosynthetic process; cellular lipid metabolic process; estrogen biosynthetic process; fatty acid biosynthetic process
NCBI Summary:	This gene encodes a very important 17beta-hydroxysteroid dehydrogenase (17beta-HSD) that converts estrone into estradiol in ovarian tissue. This enzyme is also involved in fatty acid elongation. [provided by RefSeq, Oct 2011]
UniProt Code:	Q53GQ0
NCBI GenInfo Identifier:	7705855
NCBI Gene ID:	51144
NCBI Accession:	NP_057226.1
UniProt Secondary Accession:	Q53GQ0,Q96JU2, Q9Y6G8, A8K9B0, D3DR23,
UniProt Related Accession:	Q53GQ0
Molecular Weight:	34,324 Da
NCBI Full Name:	estradiol 17-beta-dehydrogenase 12
NCBI Synonym Full Names:	hydroxysteroid (17-beta) dehydrogenase 12
NCBI Official Symbol:	HSD17B12
NCBI Official Synonym Symbols:	KAR; SDR12C1
NCBI Protein Information:	estradiol 17-beta-dehydrogenase 12; 17-beta-HSD 12; 17beta-HSD type 12; 3-ketoacyl-CoA reductase; steroid dehydrogenase homolog; 17-beta-hydroxysteroid dehydrogenase 12; short chain dehydrogenase/reductase family 12C, member 1
UniProt Protein Name:	Estradiol 17-beta-dehydrogenase 12
UniProt Synonym Protein Names:	17-beta-hydroxysteroid dehydrogenase 12; 17-beta-HSD 12; 3-ketoacyl-CoA reductase
Protein Family:	Very-long-chain 3-oxoacyl-CoA reductase
UniProt Gene Name:	HSD17B12
UniProt Entry Name:	DHB12_HUMAN

Western blot analysis of extracts of OVCAR3 cells, using HSD17B12 antibody (CAB17151) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)