Anti-HMGB3 Antibody (CAB15064)
- SKU:
- CAB15064
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Frequently bought together:
Description
| Antibody Name: | Anti-HMGB3 Antibody |
| Antibody SKU: | CAB15064 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human HMGB3 (NP_005333.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HeLa, MCF7, mouse kidney |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human HMGB3 (NP_005333.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | FFLF CSEF RPKI KSTN PGIS IGDV AKKL GEMW NNLN DSEK QPYI TKAA KLKE KYEK DVAD YKSK GKFD GAKG PAKV ARKK VEEE DEEE EEEE EEEE EEED E |
| Gene ID: | 3149 |
| Uniprot: | O15347 |
| Cellular Location: | Chromosome, Cytoplasm, Nucleus |
| Calculated MW: | 22kDa |
| Observed MW: | 23kDa |
| Synonyms: | HMGB3, HMG-2a, HMG-4, HMG2A, HMG4 |
| Background: | This gene encodes a member of a family of proteins containing one or more high mobility group DNA-binding motifs. The encoded protein plays an important role in maintaining stem cell populations, and may be aberrantly expressed in tumor cells. A mutation in this gene was associated with microphthalmia, syndromic 13. There are numerous pseudogenes of this gene on multiple chromosomes. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | HMGB3: Binds preferentially single-stranded DNA and unwinds double stranded DNA. Belongs to the HMGB family. |
| UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: Xq28 Molecular Function:DNA bending activity; double-stranded DNA binding; four-way junction DNA binding; protein binding Biological Process: DNA geometric change; DNA recombination; multicellular organismal development Disease: Microphthalmia, Syndromic 13 |
| NCBI Summary: | This gene encodes a member of a family of proteins containing one or more high mobility group DNA-binding motifs. The encoded protein plays an important role in maintaining stem cell populations, and may be aberrantly expressed in tumor cells. A mutation in this gene was associated with microphthalmia, syndromic 13. There are numerous pseudogenes of this gene on multiple chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] |
| UniProt Code: | O15347 |
| NCBI GenInfo Identifier: | 85701353 |
| NCBI Gene ID: | 3149 |
| NCBI Accession: | O15347.4 |
| UniProt Secondary Accession: | O15347,O95556, Q6NS40, |
| UniProt Related Accession: | O15347 |
| Molecular Weight: | 22,980 Da |
| NCBI Full Name: | High mobility group protein B3 |
| NCBI Synonym Full Names: | high mobility group box 3 |
| NCBI Official Symbol: | HMGB3 |
| NCBI Official Synonym Symbols: | HMG4; HMG-4; HMG2A; HMG-2a |
| NCBI Protein Information: | high mobility group protein B3 |
| UniProt Protein Name: | High mobility group protein B3 |
| UniProt Synonym Protein Names: | High mobility group protein 2a; HMG-2a; High mobility group protein 4; HMG-4 |
| Protein Family: | High mobility group protein |
| UniProt Gene Name: | HMGB3 |
 | Western blot analysis of extracts of various cell lines, using HMGB3 antibody (CAB15064) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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