Anti-HIP1 Antibody (CAB6921)
- SKU:
- CAB6921
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Frequently bought together:
Description
| Antibody Name: | Anti-HIP1 Antibody |
| Antibody SKU: | CAB6921 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 410-690 of human HIP1 (NP_005329.3). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse |
| Positive Samples: | A-549, BT-474, SW480, 293T, Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 410-690 of human HIP1 (NP_005329.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SELE ADLA EQQH LRQQ AADD CEFL RAEL DELR RQRE DTEK AQRS LSEI ERKA QANE QRYS KLKE KYSE LVQN HADL LRKN AEVT KQVS MARQ AQVD LERE KKEL EDSL ERIS DQGQ RKTQ EQLE VLES LKQE LATS QREL QVLQ GSLE TSAQ SEAN WAAE FAEL EKER DSLV SGAA HREE ELSA LRKE LQDT QLKL ASTE ESMC QLAK DQRK MLLV GSRK AAEQ VIQD ALNQ LEEP PLIS CAGS ADHL LSTV TSIS SCIE QLEK SWSQ YLAC PEDI SGLL H |
| Gene ID: | 3092 |
| Uniprot: | O00291 |
| Cellular Location: | Cytoplasm, Cytoplasmic vesicle, Endomembrane system, Nucleus, clathrin-coated vesicle membrane |
| Calculated MW: | 110kDa/113kDa/116kDa |
| Observed MW: | 120kDa |
| Synonyms: | HIP1, HIP-I, ILWEQ, SHON, SHONbeta, SHONgamma |
| Background: | The product of this gene is a membrane-associated protein that functions in clathrin-mediated endocytosis and protein trafficking within the cell. The encoded protein binds to the huntingtin protein in the brain; this interaction is lost in Huntington's disease. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | HIP1: Plays a role in clathrin-mediated endocytosis and trafficking. Involved in regulating AMPA receptor trafficking in the central nervous system in an NMDA-dependent manner. Enhances androgen receptor (AR)-mediated transcription. May act as a proapoptotic protein that induces cell death by acting through the intrinsic apoptosis pathway. Binds 3-phosphoinositides (via ENTH domain). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis. May play a functional role in the cell filament networks. May be required for differentiation, proliferation, and/or survival of somatic and germline progenitors. A chromosomal aberration involving HIP1 is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;7)(q33;q11.2) with PDGFRB. The chimeric HIP1-PDGFRB transcript results from an in-frame fusion of the two genes. The reciprocal PDGFRB-HIP1 transcript is not expressed. Belongs to the SLA2 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cytoskeletal; Apoptosis Chromosomal Location of Human Ortholog: 7q11.23 Cellular Component: Golgi apparatus; cytoskeleton; intracellular membrane-bound organelle; clathrin-coated vesicle; membrane; cytoplasm; clathrin coated vesicle membrane; nucleus Molecular Function:protein binding, bridging; clathrin binding; protein binding; structural constituent of cytoskeleton; actin binding; phosphoinositide binding Biological Process: regulation of apoptosis; caspase activation; clathrin cage assembly; transcription, DNA-dependent; regulation of transcription, DNA-dependent; apoptosis; endocytosis; cell differentiation; actin cytoskeleton organization and biogenesis; positive regulation of receptor-mediated endocytosis Disease: Prostate Cancer |
| NCBI Summary: | The product of this gene is a membrane-associated protein that functions in clathrin-mediated endocytosis and protein trafficking within the cell. The encoded protein binds to the huntingtin protein in the brain; this interaction is lost in Huntington's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
| UniProt Code: | O00291 |
| NCBI GenInfo Identifier: | 206729937 |
| NCBI Gene ID: | 3092 |
| NCBI Accession: | O00291.5 |
| UniProt Related Accession: | O00291 |
| Molecular Weight: | ~ 116kDa |
| NCBI Full Name: | Huntingtin-interacting protein 1 |
| NCBI Synonym Full Names: | huntingtin interacting protein 1 |
| NCBI Official Symbol: | HIP1 |
| NCBI Official Synonym Symbols: | SHON; HIP-I; ILWEQ; SHONbeta; SHONgamma |
| NCBI Protein Information: | huntingtin-interacting protein 1 |
| UniProt Protein Name: | Huntingtin-interacting protein 1 |
| UniProt Synonym Protein Names: | Huntingtin-interacting protein I; HIP-I |
| Protein Family: | Histidine permease |
| UniProt Gene Name: | HIP1 |
| UniProt Entry Name: | HIP1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using HIP1 antibody (CAB6921) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded mouse brain using HIP1 antibody (CAB6921) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of MCF7 cells using HIP1 antibody (CAB6921). Blue: DAPI for nuclear staining. |
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