Anti-HHAT Antibody (CAB15171)
- SKU:
- CAB15171
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-HHAT Antibody |
| Antibody SKU: | CAB15171 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HHAT (NP_060664.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A375, K-562, Mouse brain, Rat spleen |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HHAT (NP_060664.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MLPR WELA LYLL ASLG FHFY SFYE VYKV SREH EEEL DQEF ELET DTLF GGLK KDAT DFEW SFWM EWGK QWLV WLLL GHMV VSQM ATLL ARKH RPWI LMLY |
| Gene ID: | 55733 |
| Uniprot: | Q5VTY9 |
| Cellular Location: | Endoplasmic reticulum membrane, Multi-pass membrane protein |
| Calculated MW: | 9kDa/41kDa/49kDa/52kDa/56kDa/57kDa/60kDa |
| Observed MW: | 63kDa |
| Synonyms: | HHAT, MART2, SKI1, Skn |
| Background: |
| NCBI Summary: | 'Skinny hedgehog' (SKI1) encodes an enzyme that acts within the secretory pathway to catalyze amino-terminal palmitoylation of 'hedgehog' (see MIM 600725).[supplied by OMIM, Jul 2002] |
| UniProt Code: | Q5VTY9 |
| NCBI GenInfo Identifier: | 170784863 |
| NCBI Gene ID: | 55733 |
| NCBI Accession: | NP_001116306.1 |
| UniProt Related Accession: | Q5VTY9 |
| Molecular Weight: | 9kDa; 41kDa; 49kDa; 52kDa; 56kDa; 57kDa; 60kDa |
| NCBI Full Name: | protein-cysteine N-palmitoyltransferase HHAT isoform 1 |
| NCBI Synonym Full Names: | hedgehog acyltransferase |
| NCBI Official Symbol: | HHAT |
| NCBI Official Synonym Symbols: | Skn; SKI1; MART2 |
| NCBI Protein Information: | protein-cysteine N-palmitoyltransferase HHAT |
| UniProt Protein Name: | Protein-cysteine N-palmitoyltransferase HHAT |
| UniProt Synonym Protein Names: | Hedgehog acyltransferase; Melanoma antigen recognized by T-cells 2; MART-2; Skinny hedgehog protein 1 |
| Protein Family: | Protein-cysteine N-palmitoyltransferase |
| UniProt Gene Name: | HHAT |
| UniProt Entry Name: | HHAT_HUMAN |
 | Western blot analysis of extracts of various cell lines, using HHAT antibody (CAB15171) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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