Anti-HFM1 Antibody (CAB8600)
- SKU:
- CAB8600
- Product type:
- Antibody
- Application:
- WB
- Application:
- IHC
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-HFM1 Antibody |
| Antibody SKU: | CAB8600 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human HFM1 (NP_001017975.4). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | K-562 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human HFM1 (NP_001017975.4). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MLKS NDCL FSLE NLFF EKPD EVEN HPDN EKSL DWFL PPAP LISE IPDT QELE EELE SHKL LGQE KRPK MLTS NLKI TNED TNYI SLTQ KFQF AFPS DKYE QDDL NLEG VGNN DLSH VAGK LTYA SQKY KNHI GTEI APEK SVPD DTKL VNFA EDKG ESTS VFRK RLFK ISDN IHGS AYSN DNEL DSHI GSVK IVQT EMNK GKSR NYSN SKQK FQYS ANVF TANN AFSA SEIG EGMF KAPS |
| Gene ID: | 164045 |
| Uniprot: | A2PYH4 |
| Cellular Location: | |
| Calculated MW: | 62kDa/162kDa |
| Observed MW: | 130kDa |
| Synonyms: | HFM1, MER3, POF9, SEC63D1, Si-11, Si-11-6, helicase |
| Background: | The protein encoded by this gene is thought to be an ATP-dependent DNA helicase and is expressed mainly in germ-line cells. Defects in this gene are a cause of premature ovarian failure 9 (POF9). |
| UniProt Protein Function: | SEC63D1: Belongs to the helicase family. SKI2 subfamily. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:DNA-binding; EC 3.6.4.12; Helicase Chromosomal Location of Human Ortholog: 1p22.2 Molecular Function:ATP-dependent RNA helicase activity Biological Process: RNA secondary structure unwinding Disease: Premature Ovarian Failure 9 |
| NCBI Summary: | The protein encoded by this gene is thought to be an ATP-dependent DNA helicase and is expressed mainly in germ-line cells. Defects in this gene are a cause of premature ovarian failure 9 (POF9). [provided by RefSeq, Apr 2014] |
| UniProt Code: | A2PYH4 |
| NCBI GenInfo Identifier: | 206729870 |
| NCBI Gene ID: | 164045 |
| NCBI Accession: | A2PYH4.2 |
| UniProt Secondary Accession: | A2PYH4,Q8N9Q0, B1B0B6, |
| UniProt Related Accession: | A2PYH4 |
| Molecular Weight: | 163kDa |
| NCBI Full Name: | Probable ATP-dependent DNA helicase HFM1 |
| NCBI Synonym Full Names: | HFM1, ATP dependent DNA helicase homolog |
| NCBI Official Symbol: | HFM1 |
| NCBI Official Synonym Symbols: | MER3; POF9; Si-11; SEC63D1; Si-11-6; helicase |
| NCBI Protein Information: | probable ATP-dependent DNA helicase HFM1 |
| UniProt Protein Name: | Probable ATP-dependent DNA helicase HFM1 |
| UniProt Synonym Protein Names: | SEC63 domain-containing protein 1 |
| Protein Family: | ATP-dependent DNA helicase |
| UniProt Gene Name: | HFM1 |
 | Western blot analysis of extracts of K-562 cells, using HFM1 antibody (CAB8600) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat testis using HFM1 antibody (CAB8600) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse testis using HFM1 antibody (CAB8600) at dilution of 1:100 (40x lens). |
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