Anti-HEXIM2 항체 (CAB15565)

Antibody Name:	Anti-HEXIM2 Antibody
Antibody SKU:	CAB15565
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 147-286 of human HEXIM2 (NP_653209.1).

Application:	WB
Recommended Dilution:	WB 1:200 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	HepG2, HeLa, Mouse eye, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 147-286 of human HEXIM2 (NP_653209.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LMND RDPE EPNL DVPH GISH PGSS GESE AGDS DGRG RAHG EFQR KDFS ETYE RFHT ESLQ GRSK QELV RDYL ELEK RLSQ AEEE TRRL QQLQ ACTG QQSC RQVE ELAA EVQR LRTE NQRL RQEN QMWN REGC RCDE EPGT
Gene ID:	124790
Uniprot:	Q96MH2
Cellular Location:	Nucleus
Calculated MW:	32kDa
Observed MW:	45kDa

Synonyms:

HEXIM2, L3

Background:

This gene encodes a member of the HEXIM family of proteins. This protein is a component of the 7SK small nuclear ribonucleoprotein. This protein has been found to negatively regulate the kinase activity of the cyclin-dependent kinase P-TEFb, which phosphorylates multiple target proteins to promote transcriptional elongation. This gene is located approximately 7 kb downstream from related family member HEXIM1 on chromosome 17. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	HEXIM2: a transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. Component of the 7SK snRNP complex containing at least the P-TEFb complex (CDK9/CCNT1, HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs). Up-regulated by HMBA (hexamethylene bisacetamide) (at protein level). A coiled-coil domain mediates oligomerization.
UniProt Protein Details:	Protein type:Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 17q21.31 Cellular Component: cytoplasm; nucleoplasm; nucleus Molecular Function:cyclin-dependent protein kinase inhibitor activity; protein binding; snRNA binding Biological Process: negative regulation of cyclin-dependent protein kinase activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent
NCBI Summary:	This gene encodes a member of the HEXIM family of proteins. This protein is a component of the 7SK small nuclear ribonucleoprotein. This protein has been found to negatively regulate the kinase activity of the cyclin-dependent kinase P-TEFb, which phosphorylates multiple target proteins to promote transcriptional elongation. This gene is located approximately 7 kb downstream from related family member HEXIM1 on chromosome 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]
UniProt Code:	Q96MH2
NCBI GenInfo Identifier:	74732374
NCBI Gene ID:	124790
NCBI Accession:	Q96MH2.1
UniProt Secondary Accession:	Q96MH2,D3DX66,
UniProt Related Accession:	Q96MH2
Molecular Weight:	32,419 Da
NCBI Full Name:	Protein HEXIM2
NCBI Synonym Full Names:	hexamethylene bisacetamide inducible 2
NCBI Official Symbol:	HEXIM2
NCBI Official Synonym Symbols:	L3
NCBI Protein Information:	protein HEXIM2
UniProt Protein Name:	Protein HEXIM2
UniProt Synonym Protein Names:	Hexamethylene bis-acetamide-inducible protein 2
Protein Family:	Protein
UniProt Gene Name:	HEXIM2
UniProt Entry Name:	HEXI2_HUMAN

Western blot analysis of extracts of various cell lines, using HEXIM2 antibody (CAB15565) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)