Anti-HEXIM1 Antibody (CAB5775)
- SKU:
- CAB5775
- Product type:
- Antibody
- Reactivity:
- Human
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Frequently bought together:
Description
| Antibody Name: | Anti-HEXIM1 Antibody |
| Antibody SKU: | CAB5775 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 210-359 of human HEXIM1 (NP_006451.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Rat |
| Positive Samples: | SW480, HepG2, 293T, MCF7, HeLa, HL-60 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 210-359 of human HEXIM1 (NP_006451.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MDDH DQEE PDLK TGLY SKRA AAKS DDTS DDDF MEEG GEED GGSD GMGG DGSE FLQR DFSE TYER YHTE SLQN MSKQ ELIK EYLE LEKC LSRM EDEN NRLR LESK RLGG DDAR VREL ELEL DRLR AENL QLLT ENEL HRQQ ERAP LSKF GD |
| Gene ID: | 10614 |
| Uniprot: | O94992 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 40kDa |
| Observed MW: | 70kDa |
| Synonyms: | HEXIM1, CLP1, EDG1, HIS1, MAQ1 |
| Background: | Expression of this gene is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. This gene has no introns. |
| UniProt Protein Function: | HEXIM1: Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. Homooligomer and heterooligomer with HEXIM2; probably dimeric. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Interacts with the N-CoR complex through NCOR1. Interacts with ESR1 and NR3C1. May interact with NF-kappa-B through RELA. Up-regulated by HMBA (hexamethylene bisacetamide). Down-regulated by estrogen. Ubiquitously expressed with higher expression in placenta. HEXIM1 and HEXIM2 are differentially expressed. Expressed in endocrine tissues. Belongs to the HEXIM family. |
| UniProt Protein Details: | Protein type:Transcription factor; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 17q21.31 Cellular Component: cytoplasm; nucleoplasm; nucleus Molecular Function:cyclin-dependent protein kinase inhibitor activity; protein binding; snRNA binding Biological Process: heart development; negative regulation of cyclin-dependent protein kinase activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; transcription, DNA-dependent |
| NCBI Summary: | Expression of this gene is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. This gene has no introns. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O94992 |
| NCBI GenInfo Identifier: | 74735573 |
| NCBI Gene ID: | 10614 |
| NCBI Accession: | O94992.1 |
| UniProt Secondary Accession: | O94992,B2R8Y5, |
| UniProt Related Accession: | O94992 |
| Molecular Weight: | 40,623 Da |
| NCBI Full Name: | Protein HEXIM1 |
| NCBI Synonym Full Names: | hexamethylene bis-acetamide inducible 1 |
| NCBI Official Symbol: | HEXIM1 |
| NCBI Official Synonym Symbols: | CLP1; EDG1; HIS1; MAQ1 |
| NCBI Protein Information: | protein HEXIM1 |
| UniProt Protein Name: | Protein HEXIM1 |
| UniProt Synonym Protein Names: | Cardiac lineage protein 1; Estrogen down-regulated gene 1 protein; Hexamethylene bis-acetamide-inducible protein 1; Menage a quatre protein 1 |
| Protein Family: | Protein |
| UniProt Gene Name: | HEXIM1 |
| UniProt Entry Name: | HEXI1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using HEXIM1 antibody (CAB5775) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded rat brain using HEXIM1 Antibody (CAB5775) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat brain using HEXIM1 Antibody (CAB5775) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human colon cancer using HEXIM1 Antibody (CAB5775) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of A549 cells using HEXIM1 antibody (CAB5775). Blue: DAPI for nuclear staining. |
Related Products
