Anti-HELLS Antibody (CAB5831)[KO Validated]
- SKU:
- CAB5831
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
Antibody Name: | Anti-HELLS Antibody [KO Validated] |
Antibody SKU: | CAB5831 |
Antibody Size: | 20uL, 50uL, 100uL |
Application: | WB IHC IP |
Reactivity: | Human, Mouse, Rat |
Host Species: | Rabbit |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 719-838 of human HELLS (NP_060533.2). |
Application: | WB IHC IP |
Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:200 |
Reactivity: | Human, Mouse, Rat |
Positive Samples: | MCF-7, A549, 293T, HepG2, Mouse thymus |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 719-838 of human HELLS (NP_060533.2). |
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Isotype: | IgG |
Sequence: | VYRL VTAN TIDQ KIVE RAAA KRKL EKLI IHKN HFKG GQSG LNLS KNFL DPKE LMEL LKSR DYER EIKG SREK VISD KDLE LLLD RSDL IDQM NASG PIKE KMGI FKIL ENSE DSSP ECLF |
Gene ID: | 3070 |
Uniprot: | Q9NRZ9 |
Cellular Location: | Nucleus |
Calculated MW: | 14kDa/36-39kDa/81-97kDa |
Observed MW: | 120kDa |
Synonyms: | HELLS, ICF4, LSH, Nbla10143, PASG, SMARCA6 |
Background: | This gene encodes a lymphoid-specific helicase. Other helicases function in processes involving DNA strand separation, including replication, repair, recombination, and transcription. This protein is thought to be involved with cellular proliferation and may play a role in leukemogenesis. Alternatively spliced transcript variants encoding different isoforms have been identified. |
UniProt Protein Function: | HELLS: Plays an essential role in normal development and survival. Involved in regulation of the expansion or survival of lymphoid cells. Required for de novo or maintenance DNA methylation. May control silencing of the imprinted CDKN1C gene through DNA methylation. May play a role in formation and organization of heterochromatin, implying a functional role in the regulation of transcription and mitosis. By concanavalin-A in peripheral blood leukocytes. Highly expressed in proliferative tissues such as adult thymus and testis, and expressed at lower levels in uterus, small intestine, colon, and peripheral blood mononuclear cells. Also expressed in neoplastic cell lines including those derived from myeloid and lymphoid leukemias. Belongs to the SNF2/RAD54 helicase family. 9 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:EC 3.6.1.-; EC 3.6.4.-; Helicase; Apoptosis Chromosomal Location of Human Ortholog: 10q24.2 Cellular Component: centric heterochromatin; nucleus; chromosome, pericentric region Molecular Function:protein binding; DNA binding; helicase activity; ATP binding Biological Process: mitosis; lymphocyte proliferation; transcription, DNA-dependent; methylation-dependent chromatin silencing; maintenance of DNA methylation; cell division; multicellular organismal development; centric heterochromatin formation |
NCBI Summary: | This gene encodes a lymphoid-specific helicase. Other helicases function in processes involving DNA strand separation, including replication, repair, recombination, and transcription. This protein is thought to be involved with cellular proliferation and may play a role in leukemogenesis. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jan 2014] |
UniProt Code: | Q9NRZ9 |
NCBI GenInfo Identifier: | 74761670 |
NCBI Gene ID: | 3070 |
NCBI Accession: | Q9NRZ9.1 |
UniProt Secondary Accession: | Q9NRZ9,Q3LID1, Q6I7N7, Q76H76, Q76H77, Q76H78, Q76H79 Q76H80, Q76H81, Q7Z397, Q7Z5X2, B2RB41, |
UniProt Related Accession: | Q9NRZ9 |
Molecular Weight: | 838 |
NCBI Full Name: | Lymphoid-specific helicase |
NCBI Synonym Full Names: | helicase, lymphoid-specific |
NCBI Official Symbol: | HELLS |
NCBI Official Synonym Symbols: | LSH; PASG; SMARCA6; Nbla10143 |
NCBI Protein Information: | lymphoid-specific helicase; HELLS; proliferation-associated SNF2-like protein; SWI/SNF2-related matrix-associated actin-dependent regulator of chromatin subfamily A member 6; SWI/SNF2-related, matrix-associated, actin-dependent regulator of chromatin, subfamily A, member 6 |
UniProt Protein Name: | Lymphoid-specific helicase |
UniProt Synonym Protein Names: | Proliferation-associated SNF2-like protein; SWI/SNF2-related matrix-associated actin-dependent regulator of chromatin subfamily A member 6 |
Protein Family: | Lymphoid-specific helicase |
UniProt Gene Name: | HELLS |
UniProt Entry Name: | HELLS_HUMAN |