Anti-HCRTR1 Antibody (CAB14740)
- SKU:
- CAB14740
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-HCRTR1 Antibody |
| Antibody SKU: | CAB14740 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 150-250 of human HCRTR1 (NP_001516.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse brain, Rat brain |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 150-250 of human HCRTR1 (NP_001516.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | HPLL FKST ARRA RGSI LGIW AVSL AIMV PQAA VMEC SSVL PELA NRTR LFSV CDER WADD LYPK IYHS CFFI VTYL APLG LMAM AYFQ IFRK LWGR QIPG T |
| Gene ID: | 3061 |
| Uniprot: | O43613 |
| Cellular Location: | Cell membrane, Multi-pass membrane protein |
| Calculated MW: | 47kDa |
| Observed MW: | 47kDa |
| Synonyms: | HCRTR1, OX1R |
| Background: | The protein encoded by this gene is a G-protein coupled receptor involved in the regulation of feeding behavior. The encoded protein selectively binds the hypothalamic neuropeptide orexin A. A related gene (HCRTR2) encodes a G-protein coupled receptor that binds orexin A and orexin B. |
| UniProt Protein Function: | HCRTR1: Moderately selective excitatory receptor for orexin-A and, with a lower affinity, for orexin-B neuropeptide. Seems to be exclusively coupled to the G(q) subclass of heteromeric G proteins, which activates the phospholipase C mediated signaling cascade. Belongs to the G-protein coupled receptor 1 family. |
| UniProt Protein Details: | Protein type:Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral Chromosomal Location of Human Ortholog: 1p33 Cellular Component: integral to plasma membrane; plasma membrane Molecular Function:G-protein coupled receptor activity; neuropeptide receptor activity; orexin receptor activity; peptide binding Biological Process: cellular response to hormone stimulus; regulation of circadian sleep/wake cycle, sleep; synaptic transmission |
| NCBI Summary: | The protein encoded by this gene is a G-protein coupled receptor involved in the regulation of feeding behavior. The encoded protein selectively binds the hypothalamic neuropeptide orexin A. A related gene (HCRTR2) encodes a G-protein coupled receptor that binds orexin A and orexin B. [provided by RefSeq, Jan 2009] |
| UniProt Code: | O43613 |
| NCBI GenInfo Identifier: | 205804177 |
| NCBI Gene ID: | 3061 |
| NCBI Accession: | O43613.2 |
| UniProt Secondary Accession: | O43613,Q9HBV6, A8K3A6, |
| UniProt Related Accession: | O43613 |
| Molecular Weight: | 47,536 Da |
| NCBI Full Name: | Orexin receptor type 1 |
| NCBI Synonym Full Names: | hypocretin receptor 1 |
| NCBI Official Symbol: | HCRTR1 |
| NCBI Official Synonym Symbols: | OX1R |
| NCBI Protein Information: | orexin receptor type 1 |
| UniProt Protein Name: | Orexin receptor type 1 |
| UniProt Synonym Protein Names: | Hypocretin receptor type 1 |
| Protein Family: | Orexin receptor |
| UniProt Gene Name: | HCRTR1 |
| UniProt Entry Name: | OX1R_HUMAN |
 | Western blot analysis of extracts of various cell lines, using HCRTR1 antibody (CAB14740) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
 | Immunofluorescence analysis of mouse brain using HCRTR1 Rabbit pAb (CAB14740) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |