Anti-HCLS1 Antibody (CAB2165)

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SKU:
CAB2165
Product type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
Frequently bought together:

Description

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Antibody Name:Anti-HCLS1 Antibody
Antibody SKU:CAB2165
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human HCLS1 (NP_005326.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:BT-474, Jurkat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human HCLS1 (NP_005326.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MWKS VVGH DVSV SVET QGDD WDTD PDFV NDIS EKEQ RWGA KTIE GSGR TEHI NIHQ LRNK VSEE HDVL RKKE MESG PKAS HGYG GRFG VERD RMDK SAVG HEYV AEVE KHSS QTDA AKGF GGKY GVER DRAD KSAV GFDY KGEV EKHT SQKD YSRG FGGR YGVE KDKW DKAA LGYD YKGE TEKH ESQR DYAK GFGG QYGI QKDR VDKS AVGF NEME APTT AYKK TTPI EAAS SGTR GLKA KFES MAEE KRKR EEEE KAQQ VARR QQER KAVT KRSP EAPQ
Gene ID:3059
Uniprot:P14317
Cellular Location:Cytoplasm, Membrane, Mitochondrion, Peripheral membrane protein
Calculated MW:24kDa/54kDa
Observed MW:72kDa
Synonyms:HCLS1, CTTNL, HS1, lckBP1, p75
Background:
UniProt Protein Function:HS1: Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression. Associates with the SH2 and SH3 domains of LCK. Binding to he LCK SH3 domain occurs constitutively, while binding to the LCK SH2 domain occurs only upon TCR stimulation. A similar binding pattern was observed with LYN, but not with FYN in which the FYN SH2 region associates upon TCR stimulation but the FYN SH3 region does not associate regardless of TCR stimulation. Directly associates with HAX1, through binding to its C-terminal region. Interacts with HS1BP3. Interacts with FES/FPS. Interacts (via SH2 domain) with FGR. Forms a multiprotein complex with LYN and ANKRD54. Expressed only in tissues and cells of hematopoietic origin.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 3q13

Cellular Component: cytoplasm; nucleus; transcription factor complex

Molecular Function:protein binding; protein kinase binding

Biological Process: erythrocyte differentiation; positive regulation of cell proliferation; positive regulation of granulocyte differentiation; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of transcription factor import into nucleus; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tyrosine phosphorylation of STAT protein; regulation of actin filament polymerization; regulation of transcription, DNA-dependent; response to hormone stimulus

UniProt Code:P14317
NCBI GenInfo Identifier:317373440
NCBI Gene ID:3059
NCBI Accession:P14317.3
UniProt Secondary Accession:P14317,Q53Y93, Q6IBK9, Q9UDK0, B4DQ69,
UniProt Related Accession:P14317
Molecular Weight:24,008 Da
NCBI Full Name:Hematopoietic lineage cell-specific protein
NCBI Synonym Full Names:hematopoietic cell-specific Lyn substrate 1
NCBI Official Symbol:HCLS1  
NCBI Official Synonym Symbols:HS1; p75; CTTNL; lckBP1  
NCBI Protein Information:hematopoietic lineage cell-specific protein
UniProt Protein Name:Hematopoietic lineage cell-specific protein
UniProt Synonym Protein Names:Hematopoietic cell-specific LYN substrate 1; LckBP1; p75
Protein Family:HCLS1-binding protein
UniProt Gene Name:HCLS1  
UniProt Entry Name:HCLS1_HUMAN
Anti-HCLS1 Antibody (CAB2165)Western blot analysis of extracts of various cell lines, using HCLS1 antibody (CAB2165) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-HCLS1 Antibody (CAB2165)Immunohistochemistry of paraffin-embedded human liver cancer using HCLS1 antibody (CAB2165) at dilution of 1:100 (40x lens).
Anti-HCLS1 Antibody (CAB2165)Immunohistochemistry of paraffin-embedded human esophagus using HCLS1 antibody (CAB2165) at dilution of 1:100 (40x lens).
Anti-HCLS1 Antibody (CAB2165)Immunohistochemistry of paraffin-embedded mouse kidney using HCLS1 antibody (CAB2165) at dilution of 1:100 (40x lens).

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