Anti-HAUS8 Antibody (CAB7847)
- SKU:
- CAB7847
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| Antibody Name: | Anti-HAUS8 Antibody |
| Antibody SKU: | CAB7847 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 166-410 of human HAUS8 (NP_219485.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | Jurkat, NCI-H460, Mouse testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 166-410 of human HAUS8 (NP_219485.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | NLAE FERR AEKN LLIM CKEK EKLQ KKAH ELKR RLLL SQRK RELA DVLD AQIE MLSP FEAV ATRF KEQY RTFA TALD TTRH ELPV RSIH LEGD GQQL LDAL QHEL VTTQ RLLG ELDV GDSE ENVQ VLDL LSEL KDVT AKKD LELR RSFA QVLE LSAE ASKE AALA NQEV WEET QGMA PPSR WYFN QDSA CRES GGAP KNTP LSED DNPG ASSA PAQA TFIS PSED FSSS SQAE VPPS LSRS GRDL S |
| Gene ID: | 93323 |
| Uniprot: | Q9BT25 |
| Cellular Location: | Cytoplasm, centrosome, cytoskeleton, microtubule organizing center, spindle, spindle pole |
| Calculated MW: | 38kDa/44kDa |
| Observed MW: | 41kDa |
| Synonyms: | HAUS8, DGT4, HICE1, NY-SAR-48 |
| Background: |
| UniProt Protein Function: | HAUS8: Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex. Belongs to the HAUS8 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell cycle regulation Chromosomal Location of Human Ortholog: 19p13.11 Cellular Component: centrosome; cytosol Biological Process: centrosome organization and biogenesis; G2/M transition of mitotic cell cycle; spindle assembly |
| NCBI Summary: | HAUS8 is 1 of 8 subunits of the 390-kD human augmin complex, or HAUS complex. The augmin complex was first identified in Drosophila, and its name comes from the Latin verb 'augmentare,' meaning 'to increase.' The augmin complex is a microtubule-binding complex involved in microtubule generation within the mitotic spindle and is vital to mitotic spindle assembly (Goshima et al., 2008 [PubMed 18443220]; Uehara et al., 2009 [PubMed 19369198]).[supplied by OMIM, Jun 2010] |
| UniProt Code: | Q9BT25 |
| NCBI GenInfo Identifier: | 229462967 |
| NCBI Gene ID: | 93323 |
| NCBI Accession: | Q9BT25.3 |
| UniProt Secondary Accession: | Q9BT25,Q49AC4, Q86WF0, Q96FX3, B4DJA7, C9JBZ4, |
| UniProt Related Accession: | Q9BT25 |
| Molecular Weight: | 44,786 Da |
| NCBI Full Name: | HAUS augmin-like complex subunit 8 |
| NCBI Synonym Full Names: | HAUS augmin like complex subunit 8 |
| NCBI Official Symbol: | HAUS8 |
| NCBI Official Synonym Symbols: | DGT4; HICE1; NY-SAR-48 |
| NCBI Protein Information: | HAUS augmin-like complex subunit 8 |
| UniProt Protein Name: | HAUS augmin-like complex subunit 8 |
| UniProt Synonym Protein Names: | HEC1/NDC80-interacting centrosome-associated protein 1; Sarcoma antigen NY-SAR-48 |
| Protein Family: | HAUS augmin-like complex |
| UniProt Gene Name: | HAUS8 |
| UniProt Entry Name: | HAUS8_HUMAN |
 | Western blot analysis of extracts of various cell lines, using HAUS8 antibody (CAB7847) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of U2OS cells using HAUS8 antibody (CAB7847) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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