Anti-HAT1 Antibody (CAB6188)
- SKU:
- CAB6188
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-HAT1 Antibody |
| Antibody SKU: | CAB6188 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF IP ChIP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 245-419 of human HAT1 (NP_003633.1). |
| Application: | WB IHC IF IP ChIP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 ChIP 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, SW480, Jurkat, 293T, HeLa, HepG2, U-251MG, Mouse spleen, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 245-419 of human HAT1 (NP_003633.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TPFQ GQGH GAQL LETV HRYY TEFP TVLD ITAE DPSK SYVK LRDF VLVK LCQD LPCF SREK LMQG FNED MAIE AQQK FKIN KQHA RRVY EILR LLVT DMSD AEQY RSYR LDIK RRLI SPYK KKQR DLAK MRKC LRPE ELTN QMNQ IEIS MQHE QLEE SFQE LVED YRRV IERL AQE |
| Gene ID: | 8520 |
| Uniprot: | O14929 |
| Cellular Location: | Cytoplasm, Nucleus, Nucleus matrix, nucleoplasm |
| Calculated MW: | 39kDa/49kDa |
| Observed MW: | 50kDa |
| Synonyms: | HAT1, KAT1 |
| Background: | The protein encoded by this gene is a type B histone acetyltransferase (HAT) that is involved in the rapid acetylation of newly synthesized cytoplasmic histones, which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. Specifically, this HAT can acetylate soluble but not nucleosomal histone H4 at lysines 5 and 12, and to a lesser degree, histone H2A at lysine 5. Alternatively spliced transcript variants have been identified for this gene. |
| UniProt Protein Function: | HAT1: Acetylates soluble but not nucleosomal histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, acetylates histone H2A at 'Lys-5' (H2AK5ac). Has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG. May be involved in nucleosome assembly during DNA replication and repair as part of the histone H3.1 and H3.3 complexes. May play a role in DNA repair in response to free radical damage. Belongs to the HAT1 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Acetyltransferase; EC 2.3.1.48 Chromosomal Location of Human Ortholog: 2q31.2-q33.1 Cellular Component: intracellular membrane-bound organelle; nuclear chromatin; nuclear chromosome, telomeric region; nucleoplasm; nucleus; protein complex Molecular Function:H4 histone acetyltransferase activity; histone acetyltransferase activity; histone binding; protein binding Biological Process: DNA packaging; DNA replication-dependent nucleosome assembly; DNA replication-independent nucleosome assembly; internal protein amino acid acetylation |
| NCBI Summary: | The protein encoded by this gene is a type B histone acetyltransferase (HAT) that is involved in the rapid acetylation of newly synthesized cytoplasmic histones, which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. Specifically, this HAT can acetylate soluble but not nucleosomal histone H4 at lysines 5 and 12, and to a lesser degree, histone H2A at lysine 5. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jun 2009] |
| UniProt Code: | O14929 |
| NCBI GenInfo Identifier: | 3334209 |
| NCBI Gene ID: | 8520 |
| NCBI Accession: | O14929.1 |
| UniProt Secondary Accession: | O14929,Q49A44, Q53QF0, Q53SU4, Q6P594, Q8WWB9, |
| UniProt Related Accession: | O14929 |
| Molecular Weight: | 39,786 Da |
| NCBI Full Name: | Histone acetyltransferase type B catalytic subunit |
| NCBI Synonym Full Names: | histone acetyltransferase 1 |
| NCBI Official Symbol: | HAT1 |
| NCBI Official Synonym Symbols: | KAT1 |
| NCBI Protein Information: | histone acetyltransferase type B catalytic subunit |
| UniProt Protein Name: | Histone acetyltransferase type B catalytic subunit |
| UniProt Synonym Protein Names: | Histone acetyltransferase 1 |
| Protein Family: | HAT1-interacting factor |
| UniProt Gene Name: | HAT1 |
| UniProt Entry Name: | HAT1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using HAT1 antibody (CAB6188) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded mouse osteosarcoma using HAT1 antibody (CAB6188) at dilution of 1:200 (40x lens). |
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