Anti-HAP1 Antibody (CAB16333)
- SKU:
- CAB16333
- Product type:
- Antibody
- Reactivity:
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Autophagy
Description
| Antibody Name: | Anti-HAP1 Antibody |
| Antibody SKU: | CAB16333 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human HAP1 (NP_817084.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse brain, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human HAP1 (NP_817084.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MRPK RLGR CCAG SRLG PGDP AALT CAPS PSAS PAPE PSAQ PQAR GTGQ RVGS RATS GSQF LSEA RTGA RPAS EAGA KAGA RRPS AFSA IQGD VRSM PDNS DAPW TRFV FQGP FGSR ATGR GTGK AAGI WKTP AAYV GRRP GVSG PERA AFIR ELEE ALCP NLPP PVKK ITQE DVKV MLYL LEEL LPPV WESV TYGM VLQR ERDL NTAA RIGQ SLVK QNSV LMEE NSKL EALL GSAK EEIL |
| Gene ID: | 9001 |
| Uniprot: | P54257 |
| Cellular Location: | |
| Calculated MW: | 66kDa/67kDa/69kDa/75kDa |
| Observed MW: | 76kDa |
| Synonyms: | HAP1, HAP2, HIP5, HLP, hHLP1 |
| Background: | Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. This gene encodes a protein that interacts with huntingtin, with two cytoskeletal proteins (dynactin and pericentriolar autoantigen protein 1), and with a hepatocyte growth factor-regulated tyrosine kinase substrate. The interactions with cytoskeletal proteins and a kinase substrate suggest a role for this protein in vesicular trafficking or organelle transport. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. |
| UniProt Protein Function: | HAP1: Associates specifically with huntingtin. This binding is enhanced by an expanded polyglutamine repeat. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Cytoskeletal Chromosomal Location of Human Ortholog: 17q21.2-q21.3 Cellular Component: synaptic vesicle; cytoskeleton; mitochondrion; axon; lysosome; endoplasmic reticulum; autophagic vacuole; inclusion body; nucleus; cell junction; actin cytoskeleton Molecular Function:protein binding; brain-derived neurotrophic factor binding Biological Process: positive regulation of neurogenesis; positive regulation of inositol-1,4,5-triphosphate receptor activity; exocytosis; nerve growth factor receptor signaling pathway; positive regulation of synaptic transmission, GABAergic; hypothalamus cell differentiation; retrograde axon cargo transport; regulation of exocytosis; synaptic transmission; protein transport; protein localization; cell projection organization and biogenesis; positive regulation of epidermal growth factor receptor signaling pathway; vesicle transport along microtubule; positive regulation of neurotrophin production; autophagy; cerebellum development; brain development; anterograde axon cargo transport |
| NCBI Summary: | Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. This gene encodes a protein that interacts with huntingtin, with two cytoskeletal proteins (dynactin and pericentriolar autoantigen protein 1), and with a hepatocyte growth factor-regulated tyrosine kinase substrate. The interactions with cytoskeletal proteins and a kinase substrate suggest a role for this protein in vesicular trafficking or organelle transport. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P54257 |
| NCBI GenInfo Identifier: | 120431743 |
| NCBI Gene ID: | 9001 |
| NCBI Accession: | NP_001073339.1 |
| UniProt Related Accession: | P54257 |
| Molecular Weight: | 74 kDa |
| NCBI Full Name: | huntingtin-associated protein 1 isoform 3 |
| NCBI Synonym Full Names: | huntingtin associated protein 1 |
| NCBI Official Symbol: | HAP1 |
| NCBI Official Synonym Symbols: | HLP; HAP2; HIP5; hHLP1 |
| NCBI Protein Information: | huntingtin-associated protein 1 |
| UniProt Protein Name: | Huntingtin-associated protein 1 |
| UniProt Synonym Protein Names: | Neuroan 1 |
| Protein Family: | Huntingtin-associated protein |
| UniProt Gene Name: | HAP1 |
| UniProt Entry Name: | HAP1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using HAP1 antibody (CAB16333) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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