Anti-GYPC Antibody (CAB1232)
- SKU:
- CAB1232
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-GYPC Antibody |
| Antibody SKU: | CAB1232 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-128 of human GYPC (NP_002092.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HepG2, HL-60, Mouse heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-128 of human GYPC (NP_002092.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MWST RSPN STAW PLSL EPDP GMAS ASTT MHTT TIAE PDPG MSGW PDGR METS TPTI MDIV VIAG VIAA VAIV LVSL LFVM LRYM YRHK GTYH TNEA KGTE FAES ADAA LQGD PALQ DAGD SSRK EYFI |
| Gene ID: | 2995 |
| Uniprot: | P04921 |
| Cellular Location: | Cell membrane, Single-pass type III membrane protein |
| Calculated MW: | 11kDa/13kDa |
| Observed MW: | 34kDa |
| Synonyms: | GYPC, CD236, CD236R, GE, GE:GPC:GPD:GYPD, GPC, GPD, GYPD, PAS-2, PAS-2 |
| Background: | Glycophorin C (GYPC) is an integral membrane glycoprotein. It is a minor species carried by human erythrocytes, but plays an important role in regulating the mechanical stability of red cells. A number of glycophorin C mutations have been described. The Gerbich and Yus phenotypes are due to deletion of exon 3 and 2, respectively. The Webb and Duch antigens, also known as glycophorin D, result from single point mutations of the glycophorin C gene. The glycophorin C protein has very little homology with glycophorins A and B. Alternate splicing results in multiple transcript variants. |
| UniProt Protein Function: | GYPC: This protein is a minor sialoglycoprotein in human erythrocyte membranes. The blood group Gerbich antigens and receptors for Plasmodium falciparum merozoites are most likely located within the extracellular domain. Glycophorin-C plays an important role in regulating the stability of red cells. Belongs to the glycophorin-C family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 2q14-q21 Cellular Component: cortical cytoskeleton; membrane; integral to plasma membrane; plasma membrane Molecular Function:protein binding Disease: Malaria, Susceptibility To; Blood Group, Gerbich System |
| NCBI Summary: | Glycophorin C (GYPC) is an integral membrane glycoprotein. It is a minor species carried by human erythrocytes, but plays an important role in regulating the mechanical stability of red cells. A number of glycophorin C mutations have been described. The Gerbich and Yus phenotypes are due to deletion of exon 3 and 2, respectively. The Webb and Duch antigens, also known as glycophorin D, result from single point mutations of the glycophorin C gene. The glycophorin C protein has very little homology with glycophorins A and B. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012] |
| UniProt Code: | P04921 |
| NCBI GenInfo Identifier: | 121407 |
| NCBI Gene ID: | 2995 |
| NCBI Accession: | P04921.1 |
| UniProt Secondary Accession: | P04921,Q53SV9, Q92642, B2R522, |
| UniProt Related Accession: | P04921 |
| Molecular Weight: | 128 |
| NCBI Full Name: | Glycophorin-C |
| NCBI Synonym Full Names: | glycophorin C (Gerbich blood group) |
| NCBI Official Symbol: | GYPC |
| NCBI Official Synonym Symbols: | GE; GPC; GPD; GYPD; CD236; PAS-2; CD236R; PAS-2' |
| NCBI Protein Information: | glycophorin-C; glycophorin-D; glycoconnectin; glycoprotein beta; sialoglycoprotein D |
| UniProt Protein Name: | Glycophorin-C |
| UniProt Synonym Protein Names: | Glycoconnectin; Glycophorin-D; GPD; Glycoprotein beta; PAS-2'; Sialoglycoprotein D; CD_antigen: CD236 |
| Protein Family: | Glycophorin |
| UniProt Gene Name: | GYPC |
| UniProt Entry Name: | GLPC_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GYPC antibody (CAB1232) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of MCF-7 cells using GYPC antibody (CAB1232). Blue: DAPI for nuclear staining. |
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