Anti-GTF3A Antibody (CAB8426)
- SKU:
- CAB8426
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-GTF3A Antibody |
| Antibody SKU: | CAB8426 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GTF3A (NP_002088.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | SW480 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GTF3A (NP_002088.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MDPP AVVA ESVS SLTI ADAF IAAG ESSA PTPP RPAL PRRF ICSF PDCS ANYS KAWK LDAH LCKH TGER PFVC DYEG CGKA FIRD YHLS RHIL THTG EKPF |
| Gene ID: | 2971 |
| Uniprot: | Q92664 |
| Cellular Location: | Nucleus |
| Calculated MW: | 38kDa/41kDa |
| Observed MW: | 38kDa |
| Synonyms: | GTF3A, AP2, TFIIIA |
| Background: | The product of this gene is a zinc finger protein with nine Cis[2]-His[2] zinc finger domains. It functions as an RNA polymerase III transcription factor to induce transcription of the 5S rRNA genes. The protein binds to a 50 bp internal promoter in the 5S genes called the internal control region (ICR), and nucleates formation of a stable preinitiation complex. This complex recruits the TFIIIC and TFIIIB transcription factors and RNA polymerase III to form the complete transcription complex. The protein is thought to be translated using a non-AUG translation initiation site in mammals based on sequence analysis, protein homology, and the size of the purified protein. |
| UniProt Protein Function: | GTF3A: Interacts with the internal control region (ICR) of approximately 50 bases within the 5S RNA genes, is required for correct transcription of these genes by RNA polymerase III. Also binds the transcribed 5S RNA's. May initiate transcription of the 5S ribosomal RNA gene and maintain the stability of transcription of other genes. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transcription initiation complex; C2H2-type zinc finger protein Chromosomal Location of Human Ortholog: 13q12.3-q13.1 Cellular Component: nucleoplasm; nucleus Biological Process: rRNA transcription; transcription from RNA polymerase III promoter |
| NCBI Summary: | The product of this gene is a zinc finger protein with nine Cis[2]-His[2] zinc finger domains. It functions as an RNA polymerase III transcription factor to induce transcription of the 5S rRNA genes. The protein binds to a 50 bp internal promoter in the 5S genes called the internal control region (ICR), and nucleates formation of a stable preinitiation complex. This complex recruits the TFIIIC and TFIIIB transcription factors and RNA polymerase III to form the complete transcription complex. The protein is thought to be translated using a non-AUG translation initiation site in mammals based on sequence analysis, protein homology, and the size of the purified protein. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q92664 |
| NCBI GenInfo Identifier: | 172045838 |
| NCBI Gene ID: | 2971 |
| NCBI Accession: | Q92664.3 |
| UniProt Secondary Accession: | Q92664,Q12963, Q13097, B7ZBK5, |
| UniProt Related Accession: | Q92664 |
| Molecular Weight: | 38,779 Da |
| NCBI Full Name: | Transcription factor IIIA |
| NCBI Synonym Full Names: | general transcription factor IIIA |
| NCBI Official Symbol: | GTF3A |
| NCBI Official Synonym Symbols: | AP2; TFIIIA |
| NCBI Protein Information: | transcription factor IIIA |
| UniProt Protein Name: | Transcription factor IIIA |
| UniProt Gene Name: | GTF3A |
| UniProt Entry Name: | TF3A_HUMAN |
 | Western blot analysis of extracts of SW480 cells, using GTF3A antibody (CAB8426) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
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