Anti-GSS Antibody (CAB14535)[KO Validated]

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SKU:
CAB14535
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
Frequently bought together:

Description

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Antibody Name:Anti-GSS Antibody [KO Validated]
Antibody SKU:CAB14535
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 373-474 of human GSS (NP_000169.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:DU145, K-562, 293T, Mouse kidney, Mouse spleen, Mouse brain, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 373-474 of human GSS (NP_000169.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:NLYG EEMV QALK QLKD SEER ASYI LMEK IEPE PFEN CLLR PGSP ARVV QCIS ELGI FGVY VRQE KTLV MNKH VGHL LRTK AIEH ADGG VAAG VAVL DNPY PV
Gene ID:2937
Uniprot:P48637
Cellular Location:
Calculated MW:40kDa/52kDa
Observed MW:54kDa
Synonyms:GSS, GSHS, HEL-S-64p, HEL-S-88n
Background:Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency.
UniProt Protein Function:GSS: Defects in GSS are the cause of glutathione synthetase deficiency (GSS deficiency); also known as 5- oxoprolinuria or pyroglutamic aciduria. It is a severe form characterized by an increased rate of hemolysis and defective function of the central nervous system. Defects in GSS are the cause of glutathione synthetase deficiency of erythrocytes (GLUSYNDE)[MIM:231900]. Glutathione synthetase deficiency of erythrocytes is a mild form causing hemolytic anemia. Belongs to the eukaryotic GSH synthase family.
UniProt Protein Details:

Protein type:EC 6.3.2.3; Other Amino Acids Metabolism - glutathione; Ligase

Chromosomal Location of Human Ortholog: 20q11.2

Cellular Component: cytosol

Molecular Function:protein homodimerization activity; glutathione binding; glycine binding; magnesium ion binding; glutathione synthase activity; ATP binding

Biological Process: amino acid metabolic process; response to nutrient levels; nervous system development; response to cadmium ion; xenobiotic metabolic process; glutathione biosynthetic process; response to oxidative stress; response to amino acid stimulus; aging

Disease: Glutathione Synthetase Deficiency; Glutathione Synthetase Deficiency Of Erythrocytes, Hemolytic Anemia Due To

NCBI Summary:Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency. [provided by RefSeq, Jul 2008]
UniProt Code:P48637
NCBI GenInfo Identifier:1346191
NCBI Gene ID:2937
NCBI Accession:P48637.1
UniProt Secondary Accession:P48637,Q4TTD9, B2R697, B6F210, E1P5P9,
UniProt Related Accession:P48637
Molecular Weight:474
NCBI Full Name:Glutathione synthetase
NCBI Synonym Full Names:glutathione synthetase
NCBI Official Symbol:GSS  
NCBI Official Synonym Symbols:GSHS; HEL-S-64p; HEL-S-88n  
NCBI Protein Information:glutathione synthetase; GSH-S; GSH synthetase; glutathione synthase; epididymis secretory sperm binding protein Li 64p; epididymis secretory sperm binding protein Li 88n
UniProt Protein Name:Glutathione synthetase
UniProt Synonym Protein Names:Glutathione synthase
Protein Family:Glutathione synthetase
UniProt Gene Name:GSS  
UniProt Entry Name:GSHB_HUMAN
Anti-GSS Antibody [KO Validated] (CAB14535)Western blot analysis of extracts of various cell lines, using GSS antibody (CAB14535) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
Anti-GSS Antibody [KO Validated] (CAB14535)Western blot analysis of extracts from normal (control) and GSS knockout (KO) 293T cells, using GSS antibody (CAB14535) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

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