Anti-GRAP Antibody (CAB15391)
- SKU:
- CAB15391
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-GRAP Antibody |
| Antibody SKU: | CAB15391 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 128-217 of human GRAP (NP_006604.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:200 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | U-251MG, A-549 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 128-217 of human GRAP (NP_006604.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LNEL VDFY RTTT IAKK RQIF LRDE EPLL KSPG ACFA QAQF DFSA QDPS QLSF RRGD IIEV LERP DPHW WRGR SCGR VGFF PRSY VQPV HL |
| Gene ID: | 10750 |
| Uniprot: | Q13588 |
| Cellular Location: | |
| Calculated MW: | 25kDa |
| Observed MW: | 36kDa |
| Synonyms: | GRAP |
| Background: | This gene encodes a member of the GRB2/Sem5/Drk family and functions as a cytoplasmic signaling protein which contains an SH2 domain flanked by two SH3 domains. The SH2 domain interacts with ligand-activated receptors for stem cell factor and erythropoietin, and facilitates the formation of a stable complex with the BCR-ABL oncoprotein. This protein also associates with the Ras guanine nucleotide exchange factor SOS1 (son of sevenless homolog 1) through its N-terminal SH3 domain. In general, it couples signals from receptor and cytoplasmic tyrosine kinases to the Ras signaling pathway. |
| NCBI Summary: | This gene encodes a member of the GRB2/Sem5/Drk family and functions as a cytoplasmic signaling protein which contains an SH2 domain flanked by two SH3 domains. The SH2 domain interacts with ligand-activated receptors for stem cell factor and erythropoietin, and facilitates the formation of a stable complex with the BCR-ABL oncoprotein. This protein also associates with the Ras guanine nucleotide exchange factor SOS1 (son of sevenless homolog 1) through its N-terminal SH3 domain. In general, it couples signals from receptor and cytoplasmic tyrosine kinases to the Ras signaling pathway. [provided by RefSeq, Jul 2012] |
| UniProt Code: | Q13588 |
| NCBI GenInfo Identifier: | 5729856 |
| NCBI Gene ID: | 10750 |
| NCBI Accession: | NP_006604.1 |
| UniProt Related Accession: | Q13588 |
| Molecular Weight: | 36kDa |
| NCBI Full Name: | GRB2-related adapter protein isoform 1 |
| NCBI Synonym Full Names: | GRB2 related adaptor protein |
| NCBI Official Symbol: | GRAP |
| NCBI Official Synonym Symbols: | DFNB114 |
| NCBI Protein Information: | GRB2-related adapter protein |
| UniProt Protein Name: | GRB2-related adapter protein |
| Protein Family: | GRB2-related adapter protein |
| UniProt Gene Name: | GRAP |
| UniProt Entry Name: | GRAP_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GRAP antibody (CAB15391) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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