Anti-GPT Antibody (CAB2814)
- SKU:
- CAB2814
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-GPT Antibody |
| Antibody SKU: | CAB2814 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human GPT (NP_005300.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:200 - 1:1000 IHC 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse liver, Mouse lung, Mouse intestine, Rat liver, Rat heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human GPT (NP_005300.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MASS TGDR SQAV RHGL RAKV LTLD GMNP RVRR VEYA VRGP IVQR ALEL EQEL RQGV KKPF TEVI RANI GDAQ AMGQ RPIT FLRQ VLAL CVNP DLLS SPNF PDDA KKRA ERIL QACG GHSL GAYS VSSG IQLI REDV ARYI ERRD GGIP ADPN NVFL STGA SDAI VTVL KLLV AGEG HTRT GVLI PIPQ YP |
| Gene ID: | 2875 |
| Uniprot: | P24298 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 54kDa |
| Observed MW: | 55kDa |
| Synonyms: | GPT, AAT1, ALT1, GPT1 |
| Background: | This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase. |
| UniProt Protein Function: | GPT: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily. |
| UniProt Protein Details: | Protein type:EC 2.6.1.2; Transferase; Amino Acid Metabolism - alanine, aspartate and glutamate Chromosomal Location of Human Ortholog: 8q24.3 Cellular Component: cytosol Molecular Function:alanine transaminase activity Biological Process: amino acid biosynthetic process |
| NCBI Summary: | This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.[provided by RefSeq, Nov 2009] |
| UniProt Code: | P24298 |
| NCBI GenInfo Identifier: | 46577683 |
| NCBI Gene ID: | 2875 |
| NCBI Accession: | P24298.3 |
| UniProt Secondary Accession: | P24298,P78398, Q93076, B0YJ18, D3DWM7, |
| UniProt Related Accession: | P24298 |
| Molecular Weight: | 54,637 Da |
| NCBI Full Name: | Alanine aminotransferase 1 |
| NCBI Synonym Full Names: | glutamic--pyruvic transaminase |
| NCBI Official Symbol: | GPT |
| NCBI Official Synonym Symbols: | AAT1; ALT1; GPT1 |
| NCBI Protein Information: | alanine aminotransferase 1 |
| UniProt Protein Name: | Alanine aminotransferase 1 |
| UniProt Synonym Protein Names: | Glutamate pyruvate transaminase 1; GPT 1; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1 |
| Protein Family: | Alanine aminotransferase |
| UniProt Gene Name: | GPT |
| UniProt Entry Name: | ALAT1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GPT antibody (CAB2814) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human stomach using GPT antibody (CAB2814) at dilution of 1:100 (40x lens). |
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