Anti-GPR143 Antibody (CAB10568)
- SKU:
- CAB10568
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-GPR143 Antibody |
| Antibody SKU: | CAB10568 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 314-404 of human GPR143 (NP_000264.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:1000 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-251MG, BT-474, SW480, Mouse liver, Mouse kidney, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 314-404 of human GPR143 (NP_000264.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TGCS LGFQ SPRK EIQW ESLT TSAA EGAH PSPL MPHE NPAS GKVS QVGG QTSD EALS MLSE GSDA STIE IHTA SESC NKNE GDPA LPTH GDL |
| Gene ID: | 4935 |
| Uniprot: | P51810 |
| Cellular Location: | Apical cell membrane, Golgi apparatus, Lysosome membrane, Melanosome membrane, Multi-pass membrane protein |
| Calculated MW: | 43kDa |
| Observed MW: | 44kDa |
| Synonyms: | GPR143, NYS6, OA1 |
| Background: | This gene encodes a protein that binds to heterotrimeric G proteins and is targeted to melanosomes in pigment cells. This protein is thought to be involved in intracellular signal transduction mechanisms. Mutations in this gene cause ocular albinism type 1, also referred to as Nettleship-Falls type ocular albinism, a severe visual disorder. A related pseudogene has been identified on chromosome Y. |
| UniProt Protein Function: | GPR143: Receptor for tyrosine, L-DOPA and dopamine. After binding to L-DOPA, stimulates Ca(2+) influx into the cytoplasm, increases secretion of the neurotrophic factor SERPINF1 and relocalizes beta arrestin at the plasma membrane; this ligand- dependent signaling occurs through a G(q)-mediated pathway in melanocytic cells. Its activity is mediated by G proteins which activate the phosphoinositide signaling pathway. Plays also a role as an intracellular G protein-coupled receptor involved in melanosome biogenesis, organization and transport. Defects in GPR143 are the cause of albinism ocular type 1 (OA1); also known as Nettleship-Falls type ocular albinism. Form of albinism affecting only the eye. Pigment of the hair and skin is normal or only slightly diluted. Eyes may be severely affected with photophobia and reduced visual acuity. Nystagmus or strabismus are often associated. The irides and fundus are depigmented. Defects in GPR143 are the cause of Nystagmus congenital X-linked type 6 (NYS6). It is a condition defined as conjugated, spontaneous and involuntary ocular oscillations that appear at birth or during the first three months of life. Other associated features may include mildly decreased visual acuity, strabismus, astigmatism, and occasionally head nodding. Belongs to the G-protein coupled receptor OA family. |
| UniProt Protein Details: | Protein type:GPCR, OA family; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR Chromosomal Location of Human Ortholog: Xp22.2 Cellular Component: apical plasma membrane; cytoplasm; Golgi apparatus; lysosomal membrane; melanosome; melanosome membrane; membrane; plasma membrane Molecular Function:dopamine binding; G-protein coupled receptor activity; protein binding Biological Process: eye pigment biosynthetic process; G-protein coupled receptor protein signaling pathway; melanosome localization; melanosome organization and biogenesis; melanosome transport; phosphoinositide-mediated signaling; regulation of calcium-mediated signaling; signal transduction; visual perception Disease: Albinism, Ocular, Type I; Nystagmus 6, Congenital, X-linked |
| NCBI Summary: | This gene encodes a protein that binds to heterotrimeric G proteins and is targeted to melanosomes in pigment cells. This protein is thought to be involved in intracellular signal transduction mechanisms. Mutations in this gene cause ocular albinism type 1, also referred to as Nettleship-Falls type ocular albinism, a severe visual disorder. A related pseudogene has been identified on chromosome Y. [provided by RefSeq, Dec 2009] |
| UniProt Code: | P51810 |
| NCBI GenInfo Identifier: | 3219999 |
| NCBI Gene ID: | 4935 |
| NCBI Accession: | P51810.2 |
| UniProt Secondary Accession: | P51810,Q6NTI7, |
| UniProt Related Accession: | P51810 |
| Molecular Weight: | 44kDa |
| NCBI Full Name: | G-protein coupled receptor 143 |
| NCBI Synonym Full Names: | G protein-coupled receptor 143 |
| NCBI Official Symbol: | GPR143 |
| NCBI Official Synonym Symbols: | OA1; NYS6 |
| NCBI Protein Information: | G-protein coupled receptor 143 |
| UniProt Protein Name: | G-protein coupled receptor 143 |
| UniProt Synonym Protein Names: | Ocular albinism type 1 protein |
| Protein Family: | G-protein coupled receptor |
| UniProt Gene Name: | GPR143 |
 | Western blot analysis of extracts of various cell lines, using GPR143 antibody (CAB10568) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
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