Anti-GPER1 Antibody (CAB10217)
- SKU:
- CAB10217
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Description
| Antibody Name: | Anti-GPER1 Antibody |
| Antibody SKU: | CAB10217 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human GPER1 (NP_001496.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse brain, Mouse heart, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human GPER1 (NP_001496.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MDVT SQAR GVGL EMYP GTAQ PAAP NTTS PELN LSHP LLGT ALAN GTGE LSEH QQYV IGLF LSCL YTIF LF |
| Gene ID: | 2852 |
| Uniprot: | Q99527 |
| Cellular Location: | Basolateral cell membrane, Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle membrane, Early endosome, Endoplasmic reticulum membrane, Golgi apparatus, Golgi apparatus membrane, Mitochondrion membrane, Multi-pass membrane protein, Nucleus, Recycling endosome, axon, cytoskeleton, dendrite, dendritic spine membrane, perinuclear region, postsynaptic cell membrane, postsynaptic density, synapse, trans-Golgi network |
| Calculated MW: | 42kDa |
| Observed MW: | 52kDa |
| Synonyms: | GPER1, CEPR, CMKRL2, DRY12, FEG-1, GPCR-Br, GPER, GPR30, LERGU, LERGU2, LyGPR, mER, GPER1 |
| Background: | This gene is a member of the G-protein coupled receptor 1 family and encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum. The protein binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol 3, 4, 5-trisphosphate in the nucleus. This protein therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. Alternate transcriptional splice variants which encode the same protein have been characterized. |
| UniProt Protein Function: | GPER1: Receptor for estrogen. Belongs to the G-protein coupled receptor 1 family. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Motility/polarity/chemotaxis; Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1 Chromosomal Location of Human Ortholog: 7p22.3 Cellular Component: Golgi apparatus; integral to plasma membrane; early endosome; dendrite; nuclear envelope; trans-Golgi network; recycling endosome; perinuclear region of cytoplasm; cytoplasm; keratin filament; intracellular; dendritic shaft; endoplasmic reticulum membrane; cytoplasmic vesicle membrane; endoplasmic reticulum; postsynaptic density; Golgi membrane; presynaptic membrane; axon; mitochondrial membrane; presynaptic active zone; plasma membrane; nerve terminal; nucleus; cell junction Molecular Function:G-protein coupled receptor activity; protein binding; estrogen receptor activity; chromatin binding; mineralocorticoid receptor activity; steroid binding Biological Process: positive regulation of apoptosis; generation of action potential; positive regulation of caspase activity; nuclear fragmentation during apoptosis; negative regulation of DNA metabolic process; positive regulation of vasodilation; positive regulation of neurotransmitter secretion; negative regulation of leukocyte activation; negative regulation of cell proliferation; elevation of cytosolic calcium ion concentration; positive regulation of MAPKKK cascade; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of cAMP biosynthetic process; positive regulation of cell proliferation; inflammatory response; steroid hormone receptor signaling pathway; positive regulation of neurogenesis; cytosolic calcium ion homeostasis; negative regulation of lipid biosynthetic process; positive regulation of insulin secretion; positive regulation of inositol trisphosphate biosynthetic process; cell cycle; negative regulation of fat cell differentiation; apoptotic chromosome condensation; positive regulation of phosphoinositide 3-kinase cascade; G-protein coupled receptor protein signaling pathway; mineralocorticoid receptor signaling pathway; negative regulation of inflammatory response; positive regulation of G-protein coupled receptor protein signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; positive regulation of protein amino acid phosphorylation; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of cell migration |
| NCBI Summary: | This gene is a member of the G-protein coupled receptor 1 family and encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum. The protein binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. This protein therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. Alternate transcriptional splice variants which encode the same protein have been characterized. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q99527 |
| NCBI GenInfo Identifier: | 3023539 |
| NCBI Gene ID: | 2852 |
| NCBI Accession: | Q99527.1 |
| UniProt Secondary Accession: | Q99527,Q8BMP4, O08878, |
| UniProt Related Accession: | Q99527 |
| Molecular Weight: | 42,248 Da |
| NCBI Full Name: | G-protein coupled estrogen receptor 1 |
| NCBI Synonym Full Names: | G protein-coupled estrogen receptor 1 |
| NCBI Official Symbol: | GPER1 |
| NCBI Official Synonym Symbols: | mER; CEPR; GPER; DRY12; FEG-1; GPR30; LERGU; LyGPR; CMKRL2; LERGU2; GPCR-Br |
| NCBI Protein Information: | G-protein coupled estrogen receptor 1; heptahelix receptor; chemokine receptor-like 2; IL8-related receptor DRY12; membrane estrogen receptor; G protein-coupled receptor 30; chemoattractant receptor-like 2; leucine rich protein in GPR30 3'UTR; lymphocyte- |
| UniProt Protein Name: | G-protein coupled estrogen receptor 1 |
| UniProt Synonym Protein Names: | Chemoattractant receptor-like 2; Flow-induced endothelial G-protein coupled receptor 1; FEG-1; G protein-coupled estrogen receptor 1; G-protein coupled receptor 30; GPCR-Br; IL8-related receptor DRY12; Lymphocyte-derived G-protein coupled receptor; LYGPR; Membrane estrogen receptor; mER |
| UniProt Gene Name: | GPER1 |
| UniProt Entry Name: | GPER1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GPER1 antibody (CAB10217) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of C6 cells using GPER1 antibody (CAB10217) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH/3T3 cells using GPER1 antibody (CAB10217) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U2OS cells using GPER1 antibody (CAB10217) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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