Anti-GPD1 항체 (CAB5715)

Antibody Name:	Anti-GPD1 Antibody
Antibody SKU:	CAB5715
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-349 of human GPD1 (NP_005267.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse kidney, Mouse skeletal muscle, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-349 of human GPD1 (NP_005267.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MASK KVCI VGSG NWGS AIAK IVGG NAAQ LAQF DPRV TMWV FEED IGGK KLTE IINT QHEN VKYL PGHK LPPN VVAV PDVV QAAE DADI LIFV VPHQ FIGK ICDQ LKGH LKAN ATGI SLIK GVDE GPNG LKLI SEVI GERL GIPM SVLM GANI ASEV ADEK FCET TIGC KDPA QGQL LKEL MQTP NFRI TVVQ EVDT VEIC GALK NVVA VGAG FCDG LGFG DNTK AAVI RLGL MEMI AFAK LFCS GPVS SATF LESC GVAD LITT CYGG RNRK VAEA FART GKSI EQLE KELL NGQK LQGP ETAR ELYS ILQH KGLV DKFP LFMA VYKV CYEG QPVG EFIH CLQN HPEH M
Gene ID:	2819
Uniprot:	P21695
Cellular Location:	Cytoplasm
Calculated MW:	35kDa/37kDa
Observed MW:	38kDa

Synonyms:

GPD1, GPD-C, GPDH-C, HTGTI

Background:

This gene encodes a member of the NAD-dependent glycerol-3-phosphate dehydrogenase family. The encoded protein plays a critical role in carbohydrate and lipid metabolism by catalyzing the reversible conversion of dihydroxyacetone phosphate (DHAP) and reduced nicotine adenine dinucleotide (NADH) to glycerol-3-phosphate (G3P) and NAD+. The encoded cytosolic protein and mitochondrial glycerol-3-phosphate dehydrogenase also form a glycerol phosphate shuttle that facilitates the transfer of reducing equivalents from the cytosol to mitochondria. Mutations in this gene are a cause of transient infantile hypertriglyceridemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

UniProt Protein Function:	GPD1: Defects in GPD1 are a cause of hypertriglyceridemia, transient infantile (HTGTI). An autosomal recessive disorder characterized by onset of moderate to severe transient hypertriglyceridemia in infancy that normalizes with age. The hypertriglyceridemia is associated with hepatomegaly, moderately elevated transaminases, persistent fatty liver, and the development of hepatic fibrosis. Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.
UniProt Protein Details:	Protein type:Oxidoreductase; EC 1.1.1.8; Lipid Metabolism - glycerophospholipid Chromosomal Location of Human Ortholog: 12q13.12 Cellular Component: cytosol Molecular Function:glycerol-3-phosphate dehydrogenase (NAD+) activity Biological Process: phosphatidic acid biosynthetic process; triacylglycerol biosynthetic process Disease: Hypertriglyceridemia, Transient Infantile
NCBI Summary:	This gene encodes a member of the NAD-dependent glycerol-3-phosphate dehydrogenase family. The encoded protein plays a critical role in carbohydrate and lipid metabolism by catalyzing the reversible conversion of dihydroxyacetone phosphate (DHAP) and reduced nicotine adenine dinucleotide (NADH) to glycerol-3-phosphate (G3P) and NAD+. The encoded cytosolic protein and mitochondrial glycerol-3-phosphate dehydrogenase also form a glycerol phosphate shuttle that facilitates the transfer of reducing equivalents from the cytosol to mitochondria. Mutations in this gene are a cause of transient infantile hypertriglyceridemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]
UniProt Code:	P21695
NCBI GenInfo Identifier:	134047785
NCBI Gene ID:	2819
NCBI Accession:	P21695.4
UniProt Secondary Accession:	P21695,Q8N1B0, F8W1L5,
UniProt Related Accession:	P21695
Molecular Weight:	35,140 Da
NCBI Full Name:	Glycerol-3-phosphate dehydrogenase
NCBI Synonym Full Names:	glycerol-3-phosphate dehydrogenase 1
NCBI Official Symbol:	GPD1
NCBI Official Synonym Symbols:	GPD-C; HTGTI; GPDH-C
NCBI Protein Information:	glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic
UniProt Protein Name:	Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic
Protein Family:	Glycerol-3-phosphate dehydrogenase
UniProt Gene Name:	GPD1
UniProt Entry Name:	GPDA_HUMAN

	Western blot analysis of extracts of various cell lines, using GPD1 antibody (CAB5715) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded rat heart using GPD1 Antibody (CAB5715) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat heart using GPD1 Antibody (CAB5715) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human breast adipocytes using GPD1 Antibody (CAB5715) at dilution of 1:100 (40x lens).

Antibodies

GPD1 Antibody (PACO09549)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)