Anti-GPC1 Antibody (CAB13019)
- SKU:
- CAB13019
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-GPC1 Antibody |
| Antibody SKU: | CAB13019 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 290-420 of human GPC1 (NP_002072.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | NIH/3T3, Mouse brain, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 290-420 of human GPC1 (NP_002072.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | WRNL LDSM VLIT DKFW GTSG VESV IGSV HTWL AEAI NALQ DNRD TLTA KVIQ GCGN PKVN PQGP GPEE KRRR GKLA PRER PPSG TLEK LVSE AKAQ LRDV QDFW ISLP GTLC SEKM ALST ASDD RCWN GMA |
| Gene ID: | 2817 |
| Uniprot: | P35052 |
| Cellular Location: | Cell membrane, Endosome, Extracellular side, GPI-anchor, Lipid-anchor, Secreted, extracellular space |
| Calculated MW: | 31kDa/61kDa |
| Observed MW: | 62kDa |
| Synonyms: | GPC1, glypican |
| Background: | Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. |
| UniProt Protein Function: | GPC1: Cell surface proteoglycan that bears heparan sulfate. Binds, via the heparan sulfate side chains, alpha-4 (V) collagen and participates in Schwann cell myelination. May act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating (via the heparan sulfate side chains) with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. Required for proper skeletal muscle differentiation by sequestering FGF2 in lipid rafts preventing its binding to receptors (FGFRs) and inhibiting the FGF-mediated signaling. Associates (via the heparan sulfate side chains) with fibrillar APP-beta amyloid peptides in primitive and classic amyloid plaques and may be involved in the deposition of these senile plaques in the Alzheimer disease (AD) brain. Misprocessing of GPC1 is found in fibroblasts of patients with Niemann-Pick Type C1 disease. This is due to the defective deaminative degradation of heparan sulfate chains. Belongs to the glypican family. |
| UniProt Protein Details: | Protein type:Membrane protein, GPI anchor; Motility/polarity/chemotaxis; Extracellular matrix Chromosomal Location of Human Ortholog: 2q35-q37 Cellular Component: Golgi lumen; integral to plasma membrane; lipid raft; lysosomal lumen; plasma membrane; proteinaceous extracellular matrix Molecular Function:copper ion binding; fibroblast growth factor binding; laminin binding Biological Process: axon guidance; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; glycosaminoglycan metabolic process; heparan sulfate proteoglycan catabolic process; myelin formation; negative regulation of fibroblast growth factor receptor signaling pathway; retinoid metabolic process; Schwann cell differentiation |
| NCBI Summary: | Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P35052 |
| NCBI GenInfo Identifier: | 292495012 |
| NCBI Gene ID: | 2817 |
| NCBI Accession: | P35052.2 |
| UniProt Secondary Accession: | P35052,Q53QM4, B3KTD1, |
| UniProt Related Accession: | P35052 |
| Molecular Weight: | 31,931 Da |
| NCBI Full Name: | Glypican-1 |
| NCBI Synonym Full Names: | glypican 1 |
| NCBI Official Symbol: | GPC1 |
| NCBI Official Synonym Symbols: | glypican |
| NCBI Protein Information: | glypican-1 |
| UniProt Protein Name: | Glypican-1 |
| Protein Family: | Glypican |
| UniProt Gene Name: | GPC1 |
| UniProt Entry Name: | GPC1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GPC1 antibody (CAB13019) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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