Anti-GOT2 Antibody (CAB6915)
- SKU:
- CAB6915
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-GOT2 Antibody |
| Antibody SKU: | CAB6915 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-200 of human GOT2 (NP_002071.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SW620, HepG2, BT-474, Jurkat, Mouse kidney, Mouse liver, Mouse brain, Rat kidney, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-200 of human GOT2 (NP_002071.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SSWW THVE MGPP DPIL GVTE AFKR DTNS KKMN LGVG AYRD DNGK PYVL PSVR KAEA QIAA KNLD KEYL PIGG LAEF CKAS AELA LGEN SEVL KSGR FVTV QTIS GTGA LRIG ASFL QRFF KFSR DVFL PKPT WGNH TPIF RDAG MQLQ GYRY YDPK TCGF DFTG AVED ISK |
| Gene ID: | 2806 |
| Uniprot: | P00505 |
| Cellular Location: | Cell membrane, Mitochondrion matrix |
| Calculated MW: | 43kDa/47kDa |
| Observed MW: | 48kDa |
| Synonyms: | GOT2, KAT4, KATIV, KYAT4, mitAAT |
| Background: | Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Two transcript variants encoding different isoforms have been found for this gene. |
| UniProt Code: | P00505 |
| NCBI GenInfo Identifier: | 73486658 |
| NCBI Gene ID: | 2806 |
| NCBI Accession: | NP_002071.2 |
| UniProt Secondary Accession: | P00505,Q53FL3, Q9BWA3, |
| UniProt Related Accession: | P00505 |
| Molecular Weight: | 430 |
| NCBI Full Name: | aspartate aminotransferase, mitochondrial |
| NCBI Synonym Full Names: | glutamic-oxaloacetic transaminase 2, mitochondrial |
| NCBI Official Symbol: | GOT2 |
| NCBI Official Synonym Symbols: | KAT4; KATIV; mitAAT |
| NCBI Protein Information: | aspartate aminotransferase, mitochondrial; FABP-1; FABPpm; mAspAT; transaminase A; aspartate transaminase 2; fatty acid-binding protein; aspartate aminotransferase 2; kynurenine aminotransferase 4; kynurenine aminotransferase IV; glutamate oxaloacetate tr |
| UniProt Protein Name: | Aspartate aminotransferase, mitochondrial |
| UniProt Synonym Protein Names: | Fatty acid-binding protein; FABP-1; Glutamate oxaloacetate transaminase 2; Kynurenine aminotransferase 4; Kynurenine aminotransferase IV; Kynurenine--oxoglutarate transaminase 4; Kynurenine--oxoglutarate transaminase IV; Plasma membrane-associated fatty acid-binding protein; FABPpm; Transaminase A |
| UniProt Gene Name: | GOT2 |
| UniProt Entry Name: | AATM_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GOT2 antibody (CAB6915) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunohistochemistry of paraffin-embedded human liver using GOT2 Rabbit pAb (CAB6915) at dilution of 1:100 (40x lens). |
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