Anti-GNB1 Antibody (CAB1867)
- SKU:
- CAB1867
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-GNB1 Antibody |
| Antibody SKU: | CAB1867 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-340 of human GNB1 (NP_002065.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:1000 - 1:4000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HepG2, HeLa, BT-474 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-340 of human GNB1 (NP_002065.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSEL DQLR QEAE QLKN QIRD ARKA CADA TLSQ ITNN IDPV GRIQ MRTR RTLR GHLA KIYA MHWG TDSR LLVS ASQD GKLI IWDS YTTN KVHA IPLR SSWV MTCA YAPS GNYV ACGG LDNI CSIY NLKT REGN VRVS RELA GHTG YLSC CRFL DDNQ IVTS SGDT TCAL WDIE TGQQ TTTF TGHT GDVM SLSL APDT RLFV SGAC DASA KLWD VREG MCRQ TFTG HESD INAI CFFP NGNA FATG SDDA TCRL FDLR ADQE LMTY SHDN IICG ITSV SFSK SGRL LLAG YDDF NCNV WDAL KADR AGVL AGHD NRVS CLGV TDDG MAVA TGSW DSFL KIWN |
| Gene ID: | 2782 |
| Uniprot: | P62873 |
| Cellular Location: | |
| Calculated MW: | 36kDa/37kDa |
| Observed MW: | 37kDa |
| Synonyms: | GNB1, MRD42 |
| Background: | Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | G-beta 1: Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein- effector interaction. Belongs to the WD repeat G protein beta family. |
| UniProt Protein Details: | Protein type:G protein, heterotrimeric beta; G protein, heterotrimeric; G protein Chromosomal Location of Human Ortholog: 1p36.33 Cellular Component: cytosol; heterotrimeric G-protein complex; intracellular; lysosomal membrane; membrane; plasma membrane Molecular Function:GTPase activity; GTPase binding; protein binding; protein complex binding Biological Process: acetylcholine receptor signaling, muscarinic pathway; dopamine receptor, adenylate cyclase activating pathway; platelet activation; protein folding; Ras protein signal transduction; rhodopsin mediated signaling; signal transduction; Wnt receptor signaling pathway, calcium modulating pathway |
| NCBI Summary: | Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013] |
| UniProt Code: | P62873 |
| NCBI GenInfo Identifier: | 51317302 |
| NCBI Gene ID: | 2782 |
| NCBI Accession: | P62873.3 |
| UniProt Secondary Accession: | P62873,P04697, P04901, Q1RMY8, B1AJZ7, |
| UniProt Related Accession: | P62873 |
| Molecular Weight: | 36,298 Da |
| NCBI Full Name: | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 |
| NCBI Synonym Full Names: | G protein subunit beta 1 |
| NCBI Official Symbol: | GNB1 |
| NCBI Official Synonym Symbols: | MRD42 |
| NCBI Protein Information: | guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 |
| UniProt Protein Name: | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 |
| UniProt Synonym Protein Names: | Transducin beta chain 1 |
| UniProt Gene Name: | GNB1 |
| UniProt Entry Name: | GBB1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GNB1 antibody (CAB1867) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunofluorescence analysis of L929 cells using GNB1 Rabbit pAb (CAB1867) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using GNB1 Rabbit pAb (CAB1867) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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