Anti-GNAI1 Antibody (CAB8844)

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SKU:
CAB8844
Product type:
Antibody
Application:
WB
Application:
IF
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle
Frequently bought together:

Description

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Antibody Name:Anti-GNAI1 Antibody
Antibody SKU:CAB8844
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-354 of human GNAI1 (NP_002060.4).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, Mouse brain, Mouse kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-354 of human GNAI1 (NP_002060.4).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGCT LSAE DKAA VERS KMID RNLR EDGE KAAR EVKL LLLG AGES GKST IVKQ MKII HEAG YSEE ECKQ YKAV VYSN TIQS IIAI IRAM GRLK IDFG DSAR ADDA RQLF VLAG AAEE GFMT AELA GVIK RLWK DSGV QACF NRSR EYQL NDSA AYYL NDLD RIAQ PNYI PTQQ DVLR TRVK TTGI VETH FTFK DLHF KMFD VGGQ RSER KKWI HCFE GVTA IIFC VALS DYDL VLAE DEEM NRMH ESMK LFDS ICNN KWFT DTSI ILFL NKKD LFEE KIKK SPLT ICYP EYAG SNTY EEAA AYIQ CQFE DLNK RKDT KEIY THFT CATD TKNV QFVF DAVT DVII KNNL KDCG LF
Gene ID:2770
Uniprot:P63096
Cellular Location:Cell membrane, Cytoplasm, Lipid-anchor, Membrane, Nucleus, centrosome, cytoskeleton, microtubule organizing center
Calculated MW:34kDa/40kDa
Observed MW:40kDa
Synonyms:GNAI1, Gi
Background:Guanine nucleotide binding proteins are heterotrimeric signal-transducing molecules consisting of alpha, beta, and gamma subunits. The alpha subunit binds guanine nucleotide, can hydrolyze GTP, and can interact with other proteins. The protein encoded by this gene represents the alpha subunit of an inhibitory complex. The encoded protein is part of a complex that responds to beta-adrenergic signals by inhibiting adenylate cyclase. Two transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:G-alpha i1: Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division. Interacts with GPSM1. Interacts with RGS12. Interacts (via active GTP- or inactive GDP-bound forms) with RGS14 (via RGS and GoLoco domains); the interaction occurs in the centrosomes. Interacts (GDP-bound form) with RIC8A (via C-terminus). Interacts with DRD2. G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Belongs to the G-alpha family. G(i/o/t/z) subfamily.
UniProt Protein Details:

Protein type:G protein, heterotrimeric; G protein; G protein, heterotrimeric alpha G((i/o/t/z))

Chromosomal Location of Human Ortholog: 7q21

Cellular Component: centrosome; lysosomal membrane; cytoplasm; plasma membrane; heterotrimeric G-protein complex; midbody; nucleus

Molecular Function:GTPase activity; protein binding; signal transducer activity; GTP binding; metal ion binding; G-protein beta/gamma-subunit binding; metabotropic serotonin receptor binding

Biological Process: G-protein signaling, coupled to cAMP nucleotide second messenger; synaptic transmission; G-protein coupled receptor protein signaling pathway; response to peptide hormone stimulus; platelet activation; metabolic process; cell division; G-protein signaling, adenylate cyclase inhibiting pathway; blood coagulation; cell cycle

NCBI Summary:Guanine nucleotide binding proteins are heterotrimeric signal-transducing molecules consisting of alpha, beta, and gamma subunits. The alpha subunit binds guanine nucleotide, can hydrolyze GTP, and can interact with other proteins. The protein encoded by this gene represents the alpha subunit of an inhibitory complex. The encoded protein is part of a complex that responds to beta-adrenergic signals by inhibiting adenylate cyclase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
UniProt Code:P63096
NCBI GenInfo Identifier:52000964
NCBI Gene ID:2770
NCBI Accession:P63096.2
UniProt Secondary Accession:P63096,P04898, P11015, P31871, Q5U074, Q8TAN5, Q9UGA4 A8KA88, B4E2V1, C9J3A4,
UniProt Related Accession:P63096
Molecular Weight:34,775 Da
NCBI Full Name:Guanine nucleotide-binding protein G(i) subunit alpha-1
NCBI Synonym Full Names:guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1
NCBI Official Symbol:GNAI1  
NCBI Official Synonym Symbols:Gi  
NCBI Protein Information:guanine nucleotide-binding protein G(i) subunit alpha-1; guanine nucleotide-binding protein G(i) subunit alpha-1; Gi1 protein alpha subunit; adenylate cyclase-inhibiting G alpha protein
UniProt Protein Name:Guanine nucleotide-binding protein G(i) subunit alpha-1
UniProt Synonym Protein Names:Adenylate cyclase-inhibiting G alpha protein
UniProt Gene Name:GNAI1  
UniProt Entry Name:GNAI1_HUMAN
Anti-GNAI1 Polyclonal Antibody (CAB8844)Western blot analysis of extracts of various cell lines, using GNAI1 antibody (CAB8844) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

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