Anti-GMPS Antibody (CAB6606)
- SKU:
- CAB6606
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-GMPS Antibody |
| Antibody SKU: | CAB6606 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 394-693 of human GMPS (NP_003866.1). |
| Application: | WB IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IP 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF-7, SKOV3, HL-60, U-251MG, Mouse heart, Mouse kidney, Mouse brain, Rat heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 394-693 of human GMPS (NP_003866.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | DTEL IRKL REEG KVIE PLKD FHKD EVRI LGRE LGLP EELV SRHP FPGP GLAI RVIC AEEP YICK DFPE TNNI LKIV ADFS ASVK KPHT LLQR VKAC TTEE DQEK LMQI TSLH SLNA FLLP IKTV GVQG DCRS YSYV CGIS SKDE PDWE SLIF LARL IPRM CHNV NRVV YIFG PPVK EPPT DVTP TFLT TGVL STLR QADF EAHN ILRE SGYA GKIS QMPV ILTP LHFD RDPL QKQP SCQR SVVI RTFI TSDF MTGI PATP GNEI PVEV VLKM VTEI KKIP GISR IMYD LTSK PPGT TEWE |
| Gene ID: | 8833 |
| Uniprot: | P49915 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 65kDa/76kDa |
| Observed MW: | 80kDa |
| Synonyms: | GMPS, GATD7 |
| Background: | In the de novo synthesis of purine nucleotides, IMP is the branch point metabolite at which point the pathway diverges to the synthesis of either guanine or adenine nucleotides. In the guanine nucleotide pathway, there are 2 enzymes involved in converting IMP to GMP, namely IMP dehydrogenase (IMPD1), which catalyzes the oxidation of IMP to XMP, and GMP synthetase, which catalyzes the amination of XMP to GMP. |
| UniProt Protein Function: | GMPS: Involved in the de novo synthesis of guanine nucleotides which are not only essential for DNA and RNA synthesis, but also provide GTP, which is involved in a number of cellular processes important for cell division. A chromosomal aberration involving GMPS is found in acute myeloid leukemias. Translocation t(3,11)(q25,q23) with MLL. |
| UniProt Protein Details: | Protein type:Oncoprotein; Nucleotide Metabolism - purine; Xenobiotic Metabolism - drug metabolism - other enzymes; Ligase; EC 6.3.5.2 Chromosomal Location of Human Ortholog: 3q24 Cellular Component: cytoplasm; cytosol Molecular Function:GMP synthase (glutamine-hydrolyzing) activity; GMP synthase activity Biological Process: purine base biosynthetic process; purine ribonucleoside monophosphate biosynthetic process Disease: Leukemia, Acute Myeloid |
| NCBI Summary: | In the de novo synthesis of purine nucleotides, IMP is the branch point metabolite at which point the pathway diverges to the synthesis of either guanine or adenine nucleotides. In the guanine nucleotide pathway, there are 2 enzymes involved in converting IMP to GMP, namely IMP dehydrogenase (IMPD1), which catalyzes the oxidation of IMP to XMP, and GMP synthetase, which catalyzes the amination of XMP to GMP. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P49915 |
| NCBI GenInfo Identifier: | 1708072 |
| NCBI Gene ID: | 8833 |
| NCBI Accession: | P49915.1 |
| UniProt Secondary Accession: | P49915,A8K639, B4DXV7, F8W720, |
| UniProt Related Accession: | P49915 |
| Molecular Weight: | 65,929 Da |
| NCBI Full Name: | GMP synthase |
| NCBI Synonym Full Names: | guanine monophosphate synthase |
| NCBI Official Symbol: | GMPS |
| NCBI Protein Information: | GMP synthase [glutamine-hydrolyzing] |
| UniProt Protein Name: | GMP synthase [glutamine-hydrolyzing] |
| UniProt Synonym Protein Names: | GMP synthetase; Glutamine amidotransferase |
| UniProt Gene Name: | GMPS |
| UniProt Entry Name: | GUAA_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GMPS antibody (CAB6606) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug GMPS antibody (CAB6606). Western blot was performed from the immunoprecipitate using GMPS antibody (CAB6606) at a dilition of 1:1000. |
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