Anti-GMFB Antibody (CAB8652)
- SKU:
- CAB8652
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-GMFB Antibody |
| Antibody SKU: | CAB8652 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human GMFB (NP_004115.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:200 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | LO2, U-87MG, MCF7, Mouse brain, Mouse kidney, Mouse liver, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human GMFB (NP_004115.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSES LVVC DVAE DLVE KLRK FRFR KETN NAAI IMKI DKDK RLVV LDEE LEGI SPDE LKDE LPER QPRF IVYS YKYQ HDDG RVSY PLCF IFSS PVGC KPEQ QMMY AGSK NKLV QTAE LTKV FEIR NTED LTEE WLRE KLGF |
| Gene ID: | 2764 |
| Uniprot: | P60983 |
| Cellular Location: | |
| Calculated MW: | 16kDa |
| Observed MW: | 17kDa |
| Synonyms: | GMFB, GMF |
| Background: |
| UniProt Protein Function: | GMF-beta: This protein causes differentiation of brain cells, stimulation of neural regeneration, and inhibition of proliferation of tumor cells. Belongs to the actin-binding proteins ADF family. GMF subfamily. |
| UniProt Protein Details: | Protein type:Inhibitor; Motility/polarity/chemotaxis; Actin-binding Chromosomal Location of Human Ortholog: 14q22.2 Molecular Function:enzyme activator activity; protein kinase inhibitor activity; signal transducer activity Biological Process: nervous system development; protein amino acid phosphorylation; signal transduction |
| UniProt Code: | P60983 |
| NCBI GenInfo Identifier: | 46577593 |
| NCBI Gene ID: | 2764 |
| NCBI Accession: | P60983.2 |
| UniProt Secondary Accession: | P60983,P17774, Q9BS35, B2R499, |
| UniProt Related Accession: | P60983 |
| Molecular Weight: | 16,713 Da |
| NCBI Full Name: | Glia maturation factor beta |
| NCBI Synonym Full Names: | glia maturation factor beta |
| NCBI Official Symbol: | GMFB |
| NCBI Official Synonym Symbols: | GMF |
| NCBI Protein Information: | glia maturation factor beta |
| UniProt Protein Name: | Glia maturation factor beta |
| Protein Family: | Glia maturation factor |
| UniProt Gene Name: | GMFB |
| UniProt Entry Name: | GMFB_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GMFB antibody (CAB8652) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of C6 cells using GMFB Rabbit pAb (CAB8652) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using GMFB Rabbit pAb (CAB8652) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using GMFB Rabbit pAb (CAB8652) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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