Anti-GLO1 Antibody (CAB1932)
- SKU:
- CAB1932
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-GLO1 Antibody |
| Antibody SKU: | CAB1932 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-184 of human GLO1 (NP_006699.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | BT-474, 22Rv1, U-937, PC-3, Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-184 of human GLO1 (NP_006699.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAEP QPPS GGLT DEAA LSCC SDAD PSTK DFLL QQTM LRVK DPKK SLDF YTRV LGMT LIQK CDFP IMKF SLYF LAYE DKND IPKE KDEK IAWA LSRK ATLE LTHN WGTE DDET QSYH NGNS DPRG FGHI GIAV PDVY SACK RFEE LGVK FVKK PDDG KMKG LAFI QDPD GYWI EILN PNKM ATLM |
| Gene ID: | 2739 |
| Uniprot: | Q04760 |
| Cellular Location: | |
| Calculated MW: | 19kDa/20kDa |
| Observed MW: | 21kDa |
| Synonyms: | GLO1, GLOD1, GLYI, HEL-S-74 |
| Background: | The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. |
| UniProt Protein Function: | GLO1: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. Involved in the regulation of TNF-induced transcriptional activity of NF- kappa-B. Belongs to the glyoxalase I family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Carbohydrate Metabolism - pyruvate; EC 4.4.1.5; Lyase Chromosomal Location of Human Ortholog: 6p21.3-p21.1 Cellular Component: cytoplasm Molecular Function:zinc ion binding; lactoylglutathione lyase activity Biological Process: regulation of transcription from RNA polymerase II promoter; glutathione metabolic process; carbohydrate metabolic process; osteoclast differentiation; methylglyoxal metabolic process; negative regulation of apoptosis Disease: Autism |
| NCBI Summary: | The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q04760 |
| NCBI GenInfo Identifier: | 134039205 |
| NCBI Gene ID: | 2739 |
| NCBI Accession: | Q04760.4 |
| UniProt Secondary Accession: | Q04760,P78375, Q59EL0, Q5TZW3, Q96FC0, Q96J41, B2R6P7 B4DDV0, |
| UniProt Related Accession: | Q04760 |
| Molecular Weight: | Calculated: 19kDa/20kDaObserved: 21kDa |
| NCBI Full Name: | Lactoylglutathione lyase |
| NCBI Synonym Full Names: | glyoxalase I |
| NCBI Official Symbol: | GLO1 |
| NCBI Official Synonym Symbols: | GLYI; GLOD1; HEL-S-74 |
| NCBI Protein Information: | lactoylglutathione lyase; glx I; aldoketomutase; methylglyoxalase; ketone-aldehyde mutase; lactoyl glutathione lyase; glyoxalase domain containing 1; epididymis secretory protein Li 74; S-D-lactoylglutathione methylglyoxal lyase |
| UniProt Protein Name: | Lactoylglutathione lyase |
| UniProt Synonym Protein Names: | Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase |
| UniProt Gene Name: | GLO1 |
| UniProt Entry Name: | LGUL_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GLO1 antibody (CAB1932) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunohistochemistry of paraffin-embedded mouse kidney using GLO1 antibody (CAB1932) at dilution of 1:200 (40x lens). |
 | Immunofluorescence analysis of A-549 cells using GLO1 antibody (CAB1932). Blue: DAPI for nuclear staining. |
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