Anti-GLI1 항체 (CAB14675)

Antibody Name:	Anti-GLI1 Antibody
Antibody SKU:	CAB14675
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 200-300 of human GLI1 (NP_001153517.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	DU145, Mouse liver, mouse brain, rat brain

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 200-300 of human GLI1 (NP_001153517.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EKPY MCEH EGCS KAFS NASD RAKH QNRT HSNE KPYV CKLP GCTK RYTD PSSL RKHV KTVH GPDA HVTK RHRG DGPL PRAP SIST VEPK RERE GGPI REES R
Gene ID:	2735
Uniprot:	P08151
Cellular Location:	Cytoplasm, Nucleus, Nucleus
Calculated MW:	104kDa/113kDa/117kDa
Observed MW:	130kDa/160kDa

Synonyms:

GLI1, GLI

Background:

This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	GLI1: Acts as a transcriptional activator. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation. Interacts with KIF7. Interacts with ZIC1; the interaction enhances transcription activation. Amplified in glioblastoma cells. Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract. Belongs to the GLI C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transcription factor; Oncoprotein; C2H2-type zinc finger protein Chromosomal Location of Human Ortholog: 12q13.2-q13.3 Cellular Component: nucleoplasm; intracellular membrane-bound organelle; cytoplasm; nucleus; cytosol Molecular Function:RNA polymerase II transcription factor activity, enhancer binding; protein binding; DNA binding; metal ion binding; microtubule binding; chromatin binding Biological Process: transcription from RNA polymerase II promoter; smoothened signaling pathway; ventral midline development; smoothened signaling pathway in regulation of granule cell precursor cell proliferation; positive regulation of transcription, DNA-dependent; digestive tract morphogenesis; notochord regression; osteoblast differentiation; cerebellar cortex morphogenesis; dorsal/ventral pattern formation; pituitary gland development; positive regulation of cell proliferation; positive regulation of smoothened signaling pathway; epidermal cell differentiation; spermatogenesis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of DNA replication; proximal/distal pattern formation; regulation of smoothened signaling pathway; lung development
NCBI Summary:	This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]
UniProt Code:	P08151
NCBI GenInfo Identifier:	121323
NCBI Gene ID:	2735
NCBI Accession:	P08151.1
UniProt Related Accession:	P08151
Molecular Weight:
NCBI Full Name:	Zinc finger protein GLI1
NCBI Synonym Full Names:	GLI family zinc finger 1
NCBI Official Symbol:	GLI1
NCBI Official Synonym Symbols:	GLI; PPD1; PAPA8
NCBI Protein Information:	zinc finger protein GLI1
UniProt Protein Name:	Zinc finger protein GLI1
UniProt Synonym Protein Names:	Glioma-associated oncogene; Oncogene GLI
Protein Family:	Zinc finger protein
UniProt Gene Name:	GLI1
UniProt Entry Name:	GLI1_HUMAN

	Western blot analysis of extracts of various cell lines, using GLI1 antibody (CAB14675) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat testis using GLI1 antibody (CAB14675) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human mammary cancer using GLI1 antibody (CAB14675) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse testis using GLI1 antibody (CAB14675) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using GLI1 antibody (CAB14675) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

Anti-GLI1 Antibody (CAB8387)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)