Anti-GFPT1 Antibody (CAB3882)
- SKU:
- CAB3882
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-GFPT1 Antibody |
| Antibody SKU: | CAB3882 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human GFPT1 (NP_002047.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:200 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, HepG2, Jurkat, HT-29, SKOV3, Mouse liver, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human GFPT1 (NP_002047.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MCGI FAYL NYHV PRTR REIL ETLI KGLQ RLEY RGYD SAGV GFDG GNDK DWEA NACK IQLI KKKG KVKA LDEE VHKQ QDMD LDIE FDVH LGIA HTRW ATHG EPSP VNSH PQRS DKNN EFIV IHNG IITN YKDL KKFL ESKG YDFE SETD TE |
| Gene ID: | 2673 |
| Uniprot: | Q06210 |
| Cellular Location: | |
| Calculated MW: | 76kDa/78kDa |
| Observed MW: | 98kDa |
| Synonyms: | GFPT1, CMS12, CMSTA1, GFA, GFAT, GFAT 1, GFAT1, GFAT1m, GFPT, GFPT1L, MSLG |
| Background: | This gene encodes the first and rate-limiting enzyme of the hexosamine pathway and controls the flux of glucose into the hexosamine pathway. The product of this gene catalyzes the formation of glucosamine 6-phosphate. |
| UniProt Protein Function: | GFAT: Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins. Homotetramer (Potential). Isoform 1 is predominantly expressed in skeletal muscle. Not expressed in brain. Seems to be selectively expressed in striated muscle. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 2.6.1.16; Amino Acid Metabolism - alanine, aspartate and glutamate; Transferase; Carbohydrate Metabolism - amino sugar and nucleotide sugar Chromosomal Location of Human Ortholog: 2p13 Cellular Component: cytosol Molecular Function:amino acid binding; carbohydrate binding; glutamine-fructose-6-phosphate transaminase (isomerizing) activity Biological Process: dolichol-linked oligosaccharide biosynthetic process; unfolded protein response; glucosamine biosynthetic process; glutamine metabolic process; post-translational protein modification; fructose 6-phosphate metabolic process; carbohydrate biosynthetic process; protein homotetramerization; response to sucrose stimulus; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; cellular response to insulin stimulus; energy reserve metabolic process; UDP-N-acetylglucosamine biosynthetic process; protein amino acid N-linked glycosylation via asparagine; circadian regulation of gene expression; negative regulation of glycogen biosynthetic process Disease: Myasthenic Syndrome, Congenital, Associated With Acetylcholine Receptor Deficiency; Myasthenic Syndrome, Congenital, With Tubular Aggregates 1 |
| NCBI Summary: | This gene encodes the first and rate-limiting enzyme of the hexosamine pathway and controls the flux of glucose into the hexosamine pathway. The product of this gene catalyzes the formation of glucosamine 6-phosphate. [provided by RefSeq, Sep 2008] |
| UniProt Code: | Q06210 |
| NCBI GenInfo Identifier: | 30923274 |
| NCBI Gene ID: | 2673 |
| NCBI Accession: | Q06210.3 |
| UniProt Related Accession: | Q06210 |
| Molecular Weight: | 98kDa |
| NCBI Full Name: | Glutamine--fructose-6-phosphate aminotransferase |
| NCBI Synonym Full Names: | glutamine--fructose-6-phosphate transaminase 1 |
| NCBI Official Symbol: | GFPT1 |
| NCBI Official Synonym Symbols: | GFA; GFAT; GFPT; MSLG; CMS12; GFAT1; CMSTA1; GFAT 1; GFAT1m; GFPT1L |
| NCBI Protein Information: | glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1 |
| UniProt Protein Name: | Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1 |
| UniProt Synonym Protein Names: | D-fructose-6-phosphate amidotransferase 1; Glutamine:fructose-6-phosphate amidotransferase 1; GFAT 1; GFAT1; Hexosephosphate aminotransferase 1 |
| Protein Family: | Glutamine--fructose-6-phosphate aminotransferase |
| UniProt Gene Name: | GFPT1 |
| UniProt Entry Name: | GFPT1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GFPT1 antibody (CAB3882) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human liver damage using GFPT1 antibody (CAB3882) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat brain using GFPT1 antibody (CAB3882) at dilution of 1:100 (40x lens). |
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