Anti-Gephyrin Antibody (CAB8572)
- SKU:
- CAB8572
- Product type:
- Antibody
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-Gephyrin Antibody |
| Antibody SKU: | CAB8572 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 520-769 of human GPHN (NP_065857.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:100 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, Mouse brain, Mouse liver, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 520-769 of human GPHN (NP_065857.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TVGV TEVE VNKF PVVA VMST GNEL LNPE DDLL PGKI RDSN RSTL LATI QEHG YPTI NLGI VGDN PDDL LNAL NEGI SRAD VIIT SGGV SMGE KDYL KQVL DIDL HAQI HFGR VFMK PGLP TTFA TLDI DGVR KIIF ALPG NPVS AVVT CNLF VVPA LRKM QGIL DPRP TIIK ARLS CDVK LDPR PEYH RCIL TWHH QEPL PWAQ STGN QMSS RLMS MRSA NGLL MLPP KTEQ YVEL HKGE VVDV MVIG RL |
| Gene ID: | 10243 |
| Uniprot: | Q9NQX3 |
| Cellular Location: | Cell junction, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, cytoskeleton, postsynaptic cell membrane, synapse |
| Calculated MW: | 79kDa/83kDa |
| Observed MW: | 103kDa |
| Synonyms: | GPHN, GEPH, GPH, GPHRYN, HKPX1, MOCODC, gephyrin |
| Background: | This gene encodes a neuronal assembly protein that anchors inhibitory neurotransmitter receptors to the postsynaptic cytoskeleton via high affinity binding to a receptor subunit domain and tubulin dimers. In nonneuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Mutations in this gene may be associated with the neurological condition hyperplexia and also lead to molybdenum cofactor deficiency. Numerous alternatively spliced transcript variants encoding different isoforms have been described; however, the full-length nature of all transcript variants is not currently known. |
| UniProt Protein Function: | gephyrin: Microtubule-associated protein involved in membrane protein-cytoskeleton interactions. It is thought to anchor the inhibitory glycine receptor (GLYR) to subsynaptic microtubules. Catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released. Homotrimer. Interacts with GABARAP. Inhibited by copper and tungsten. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 2.7.7.75; Adaptor/scaffold; EC 2.10.1.1 Chromosomal Location of Human Ortholog: 14q23.3 Cellular Component: cytoplasm; dendrite; plasma membrane; postsynaptic membrane Molecular Function:protein binding Biological Process: Mo-molybdopterin cofactor biosynthetic process; molybdenum incorporation into molybdenum-molybdopterin complex; molybdopterin cofactor biosynthetic process Disease: Hyperekplexia, Hereditary 1; Molybdenum Cofactor Deficiency, Complementation Group C |
| NCBI Summary: | This gene encodes a neuronal assembly protein that anchors inhibitory neurotransmitter receptors to the postsynaptic cytoskeleton via high affinity binding to a receptor subunit domain and tubulin dimers. In nonneuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Mutations in this gene may be associated with the neurological condition hyperplexia and also lead to molybdenum cofactor deficiency. Numerous alternatively spliced transcript variants encoding different isoforms have been described; however, the full-length nature of all transcript variants is not currently known. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9NQX3 |
| NCBI GenInfo Identifier: | 13431554 |
| NCBI Gene ID: | 10243 |
| NCBI Accession: | Q9NQX3.1 |
| UniProt Secondary Accession: | Q9NQX3,Q96KU4, Q9H4E9, Q9P2G2, |
| UniProt Related Accession: | Q9NQX3 |
| Molecular Weight: | 83,448 Da |
| NCBI Full Name: | Gephyrin |
| NCBI Synonym Full Names: | gephyrin |
| NCBI Official Symbol: | GPHN |
| NCBI Official Synonym Symbols: | GPH; GEPH; HKPX1; GPHRYN; MOCODC |
| NCBI Protein Information: | gephyrin |
| UniProt Protein Name: | Gephyrin |
| UniProt Synonym Protein Names: | Domain E |
| Protein Family: | Gephyrin |
| UniProt Gene Name: | GPHN |
| UniProt Entry Name: | GEPH_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GPHN antibody (CAB8572) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded mouse lung using GPHN antibody (CAB8572) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of rat brain using GPHN antibody (CAB8572) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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