Anti-GCKR Antibody (CAB5678)
- SKU:
- CAB5678
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-GCKR Antibody |
| Antibody SKU: | CAB5678 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GCKR (NP_001477.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HL-60, Mouse liver, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GCKR (NP_001477.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MPGT KRFQ HVIE TPEP GKWE LSGY EAAV PITE KSNP LTQD LDKA DAEN IVRL LGQC DAEI FQEE GQAL STYQ RLYS ESIL TTMV QVAG KVQE VLKE PDGG LVVL SGGG TSGR MAFL MSVS FNQL MKGL GQKP LYTY LIAG GDRS VVAS REGT EDSA LHGI EELK KVAA GKKR VIVI GISV GLSA PFVA GQMD CCMN NTAV FLPV LVGF NPVS MARN DPIE DWSS TFRQ VAER MQKM QEKQ KAFV LNPA IGPE GLSG SSRM KGGS ATKI LLET LLLA AHKT VDQG IAAS QRCL LEIL RTFE |
| Gene ID: | 2646 |
| Uniprot: | Q14397 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 16kDa/68kDa |
| Observed MW: | 60-69kDa |
| Synonyms: | GCKR, FGQTL5, GKRP |
| Background: | This gene encodes a protein belonging to the GCKR subfamily of the SIS (Sugar ISomerase) family of proteins. The gene product is a regulatory protein that inhibits glucokinase in liver and pancreatic islet cells by binding non-covalently to form an inactive complex with the enzyme. This gene is considered a susceptibility gene candidate for a form of maturity-onset diabetes of the young (MODY). |
| UniProt Protein Function: | GCKR: a key regulator of glucokinase in the liver and pancreas. Not detected in muscle, brain, heart, thymus, intestine, uterus, adipose tissue, kidney, adrenal, lung or spleen. When expressed alone, both glucokinase and GCKR localize predominantly to the cytoplasm. Co-expression induces both proteins to localize in the nucleus. |
| UniProt Protein Details: | Protein type:Inhibitor Chromosomal Location of Human Ortholog: 2p23 Cellular Component: cytoplasm; cytosol; nucleoplasm Molecular Function:protein binding Biological Process: negative regulation of glucokinase activity; protein import into nucleus, translocation; response to fructose stimulus; urate metabolic process Disease: Fasting Plasma Glucose Level Quantitative Trait Locus 5 |
| NCBI Summary: | This gene encodes a protein belonging to the GCKR subfamily of the SIS (Sugar ISomerase) family of proteins. The gene product is a regulatory protein that inhibits glucokinase in liver and pancreatic islet cells by binding non-covalently to form an inactive complex with the enzyme. This gene is considered a susceptibility gene candidate for a form of maturity-onset diabetes of the young (MODY). [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q14397 |
| NCBI GenInfo Identifier: | 327478611 |
| NCBI Gene ID: | 2646 |
| NCBI Accession: | Q14397.6 |
| UniProt Secondary Accession: | Q14397,Q53RY6, Q99522, A1L4C2, B4DPQ2, |
| UniProt Related Accession: | Q14397 |
| Molecular Weight: | 16,477 Da |
| NCBI Full Name: | Glucokinase regulatory protein |
| NCBI Synonym Full Names: | glucokinase (hexokinase 4) regulator |
| NCBI Official Symbol: | GCKR |
| NCBI Official Synonym Symbols: | GKRP; FGQTL5 |
| NCBI Protein Information: | glucokinase regulatory protein |
| UniProt Protein Name: | Glucokinase regulatory protein |
| Protein Family: | Glucokinase regulatory protein |
| UniProt Gene Name: | GCKR |
| UniProt Entry Name: | GCKR_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GCKR Antibody (CAB5678) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
Related Products
Human Glucokinase regulatory protein (GCKR) ELISA Kit
